2022年5月12日 · 首先观察Input组，第一块胶图是利用Anti-Flag检测Flag标签，结果显示第二泳道和第三泳道均检测到条带，说明第二组和第三组实验都存在A-3×Flag蛋白；第二块胶图是利用Anti-HA检测HA标签，结果显示第一泳道和第三泳道均检测到条带，说明第一组和第三组实验都存 …

Co-IP （免疫共沉淀，co-inmunoprecipitation）是经典的利用抗体从样品中捕获靶蛋白及其互作蛋白、复合体的一项技术，能够特异性富集所研究的目的蛋白。 由于过程中采用了非变性条件，保留了互作及复合体的细胞内状态。 相对于 酵母双杂交 、 pull down 等方法，其特色之一便是捕获的蛋白互作发生在所研究的特定组织细胞内，保存了互作蛋白及复合体的“内源”状态。 Co-IP实验原理： 以抗体和抗原之间的专一性作用为基础的用于研究蛋白质相互作用的经典方法，是确定 …

IPED Digital Forensic Tool. It is an open source software that can be used to process and analyze digital evidence, often seized at crime scenes by law enforcement or in a corporate investigation by private examiners.

2011年7月8日 · As part of a MudPIT-based proteomic analysis of the human Mediator complex, we generated human cell lines expressing Mediator subunits with FLAG epitope tags, immunopurified (IPed) FLAG-Mediator subunits and their associated proteins, and identified proteins in anti-FLAG eluates by MudPIT mass spectrometry.

Flag-tagged wild-type and mutant PKM2 protein were expressed in 293T cells and treated with or without NAM and TSA, then purified by IP. The PKM2 enzyme activity was measured and normalized against protein level.

2017年11月9日 · The FLAG-LETM1 proteins were isolated by IP with anti-FLAG and eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes.

2021年12月2日 · Bead-bound FLAG-GFP or IMPβ-FLAG was eluted using the FLAG peptide. To prepare IMPα–CBP80-associated mRNPs, the IPs using either α-CBP80 antibody or rIgG were performed in extracts of HEK293T cells transiently expressing a reporter mRNA (either Con-RβGl or PPL-RβGl) and either undepleted or depleted of SRβ.

2020年8月3日 · IRGM interacts directly with TFEB IRGM tagged with green fluorescent protein (GFP–IRGM) co-immunoprecipitated (co-IPed) with FLAG–TFEB (Fig. 2a).

Thus, at times it is necessary to use mammalian expressed PRMTs, which are immunoprecipitated (IPed) to serve as an enzyme source. Usually, PRMTs are fused to a Myc or HA tag, and antibodies against these tags are used to IP the PRMT from transiently transfected mammalian cells.

2011年11月8日 · The lysates were IPed using anti-Flag (left) or anti-HA (right). The IPed materials and the whole cell extracts (WCE) were analyzed on Western blot using indicated antibodies. The positions of the 120 kDa and the 100 kDa forms of IL-10R1 were marked as in (B).