2020年1月13日 · Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to engineer a...

2013年6月16日 · We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are...

2011年8月1日 · Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of...

Here we report a phytochrome-based near infra-red fluorescent protein (iRFP) with the excitation/emission maxima at 690/713 nm. Bright fluorescence in a living mouse proved iRFP to be a superior probe for non-invasive imaging of internal mammalian tissues.

2013年6月16日 · uorescent proteins (fPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared fPs—irfP670, irfP682, irfP702 and irfP720—from bacterial phytochromes. irfPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. irfP670 and irfP720 enable two-color imaging with stand...

Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore.

We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice.

Using the near-infrared fluorescent protein, iRFP713, improves the imaging resolution in thick tissue sections or the intact brain due to the reduced light-scattering at the longer, NIR wavelengths used to image the protein.

2015年1月27日 · We recently reported five NIR FPs, called iRFPs, with different fluorescence and biochemical properties. Interestingly, two of them, iRFP670 and iRFP682, exhibited the twice higher molecular...

Near-infrared fluorescent proteins for in vivo imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging.