ALK IHC and FISH discordant results in patients with NSCLC and ...
2018年9月17日 · ALK immunohistochemical (IHC) and fluorescence in situ hybridisation (FISH) discordance continues to be a challenge. Current clinical practice reveals ALK testing is not …
Discordance between FISH, IHC, and NGS Analysis of ALK Status in ...
2019年2月1日 · Immunohistochemistry (IHC) is a sensitive and specific method to detect ALK protein expression, possibly an alternative to fluorescence in situ hybridization (FISH). In this …
Anaplastic Lymphoma Kinase Testing: IHC vs. FISH vs. NGS
2017年11月16日 · This study demonstrates the feasibility of CISH as a potentially applicable ALK detection method. Overall ISH-based ALK rearrangement detection remains a reliable method …
Brief report: Clinical implications of variant ALK FISH …
Break-apart fluorescence in situ hybridization (FISH) is the FDA-approved assay for detecting anaplastic lymphoma kinase (ALK) rearrangements in non-small cell lung cancer (NSCLC), …
ALK Gene Rearrangements in Lung Adenocarcinomas: …
2021年10月1日 · Our results reveal high concordance among IHC, RNA NGS, and RNA ISH. In cases of discordance with available RNA NGS, FISH result was positive whereas IHC and ISH …
IHC versus FISH versus NGS to detect ALK gene rearrangement in …
We aimed to determine the concordance between IHC, FISH and NGS for ALK biomarker detection, and determine differences in sensitivity, and survival outcomes. Methods: We …
Multicenter ALK Testing in Non–Small-Cell Lung Cancer: Results of …
2014年10月1日 · With this study, we present the data of the first German ALK-round robin test based on genomic DNA in situ hybridization (ISH). The application of immunohistochemistry …
IHC versus FISH versus NGS to detect ALK gene rearrangement in …
Aims Anaplastic lymphoma kinase (ALK) rearranged non-small cell lung carcinoma (NSCLC) is a distinct molecular subtype and rapid approval of ALK tyrosine kinase inhibitors (TKIs) has …
Comparison of IHC, FISH and RT-PCR methods for detection of ALK ...
2013年8月7日 · Background: Recently Echinoderm microtubule-associated protein-like 4- anaplastic lymphoma kinase (EML4-ALK) fusion gene has become an important biomarker for …
ALK Gene Rearrangements in Lung Adenocarcinomas: …
2021年9月25日 · RNA NGS identified one rare and one novel ALK fusion. Sufficient therapy data were available in 10 cases treated with tyrosine kinase inhibitors; three had disease …