Thalamocortical control of cell-type specificity drives circuits for ...
2023年9月28日 · By contrast, we found that Smad7 OE (E13 IUE: Smad7/GFP) resulted in a greater number of L4 contralaterally projecting neurons in barrel cortex, consistent with there …
Translational derepression of Elavl4 isoforms at their ... - Nature
2020年4月3日 · To identify translationally-regulated isoforms, we performed an unbiased RNA-seq screen by polysome profiling of dissected wild type (WT) E13 (early neurogenesis) and …
Human TKTL1 implies greater neurogenesis in frontal ... - Science
2022年9月9日 · Using our standard approach of hTKTL1 IUE at E13.5, E14.5 neocortex was subjected to HERO culture for 24 hours in the absence or presence of the FAS inhibitor …
RGCC balances self‐renewal and neuronal ... - EMBO reports
2021年7月29日 · Using mouse in utero electroporation (IUE) technique and in vitro human NSC differentiation models including cerebral organoids (hCOs), we show here that regulator of cell …
Cortical Neural Stem Cell Lineage Progression Is Regulated by …
2020年3月31日 · To perform intersectional lineage analysis, we delivered pCAG-Cre plasmids specifically into the cortical VZ of Gsx2 Flpo/+; IS embryos on E14.5 by in utero …
Human SERPINA3 induces neocortical folding and improves
2022年11月22日 · The SERPINA3 overexpression vector was transferred into NPCs located in the ventricle zone of the mouse neocortex through in utero electroporation (IUE) at embryonic …
Lysosomal dynamics regulate mammalian cortical neurogenesis
2024年1月8日 · Injection of FT compounds and in utero electroporation (IUE) of LAMP2-mCherry plasmid enabled the labeling of the M-phase juxtaventricular RGCs and their lysosomes …
Dual in Utero Electroporation in Mice to Manipulate Two Specific ...
2022年1月11日 · IUE at embryonic day (E) 13.5 and 15.5 is targeted to neural progenitor cells that generate pyramidal neurons destined to reach L4/5 and L2/3, respectively (Molyneaux et …
Genetic fate-mapping at E13.5 separately labels Tbr2 and non …
Genetic fate-mapping at E13.5 separately labels Tbr2 and non-Tbr2 neocortical progenitor lineages. (A) A pTbr2-cre plasmid was co-transfected with a CAGstoplight reporter plasmid …
Adult Neural Stem Cells and Multiciliated Ependymal Cells Share …
2019年4月3日 · We first verified that cells targeted by in utero electroporation (IUE) are cycling by injecting EdU at E13.5 or E14.5. The next day, 78% ± 2% of electroporated cells were indeed …