Morphological changes in JEV-infected CΔ2+ cells were first observed at 48 hpi with some increased cell rounding and refractility (Figure 3C). CPE progressed in a time-dependent fashion with CΔ2+ cell detachment evident beginning at 72 hpi, and more than half of the cells appearing detached by 96 hpi . Infection-specific morphologic changes ...

2024年5月10日 · Human infections with the Japanese encephalitis virus (JEV) are a leading cause of viral encephalitis. An unprecedented outbreak of JEV genotype 4 was recently reported in...

2018年11月9日 · To identify the relationship between structure and antiviral activity of CW-33 analogues against Japanese encephalitis virus, these compounds were tested their inhibitory effect on viral cytopathic...

2024年7月15日 · We aimed to develop a reverse genetics system for Japanese encephalitis virus (JEV) genotype (GI) strain. A recombinant full-length JEV clone, pTRE-JEVGI, was generated based on a Korean wild-type JEV-GI isolate (K05GS). Rescued JEV-GI viruses were generated by transfecting BHK-21 cells with pTRE-JEVGI.

2020年10月14日 · Here, we design a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO) library containing 85,674 single guide RNAs targeting 17,743 protein-coding genes, 11,053 long ncRNAs, and 551 microRNAs....

An image-based antiviral assay for JEV was developed with the EGFP-reporter viruses, and two drugs were identified to have a broad-spectrum inhibitory effect on GI and GIII JEV replication,...

2022年11月21日 · Reverse transcription quantitative PCR (RT-qPCR) testing was performed on post-mortem brain specimens using a range of JE virus (JEV)-specific assays. Virus isolation from brain specimens was attempted by inoculation of mosquito and mammalian cells or …

Neuron-free, astrocyte-enriched brain cell cultures from newborn mice could be infected with Japanese encephalitis virus (JEV) as evidenced by immunofluorescence (IF), viral replication and cytopathic effect (CPE). Virus-specific cytoplasmic fluorescence was detectable in …

2021年11月12日 · Here, we demonstrate the susceptibility of several porcine cell lines to the attenuated genotype III JEV strain SA14-14-2. Monolayers of porcine nasal turbinate (PT-K75), kidney (SK-RST), testis (ST), and monocyte-derived macrophage (CΔ2+) cells were infected with SA14-14-2 for up to five days at a multiplicity of infection (MOI) of 0.1.

The Sf9 cell line, commonly used for gene expression by recombinant baculoviruses, can be productively infected by Japanese B encephalitis virus (JEV). Two wild-type JEV strains (P3 and SA14) caused a cytopathic effect (CPE) in the Sf9 cells, while no apparent CPE was caused by an attenuated strain …