In cis interactions between Jurkat T-cell β2 integrins and CD47 were detected by fluorescence lifetime imaging microscopy. Unexpectedly, Jurkat CD47 null cells exhibited a striking defect in β1 and β2 integrin activation in response to Mn(2+) or …

2018年2月7日 · CD63 and MHC1 are widely used as markers to purify EVs. CD47 is also present on EVs and alters their effects on target cells, suggesting that specific surface markers define functionally distinct...

Thrombospondin-1 (TSP1) inhibits angiogenesis, in part, by interacting with the ubiquitous cell-surface receptor CD47. In endothelial cells, CD47 interacts directly with vascular...

2021年12月1日 · SIRPγ and CD47 Do Not Colocalize on Jurkat, Neither on Resting nor on Proliferating T Cells. Since SIRPγ and CD47 are both expressed on T cells with a similar expression pattern on the subpopulations, we hypothesized that CD47 may sequester SIRPγ at the cell surface by cis ligation on resting T cells. We first addressed this question using ...

2022年12月27日 · In vitro binding assays revealed that IMM0306 has a high affinity for the CD47 target on Jurkat cells, the CD20 target on Raji-CD47KO cells, and the simultaneous binding of CD47/CD20 double...

2018年5月10日 · CD47-ligation is known to induce Jurkat T-ALL cell death 14,15,21,22,28. To evaluate if CC2C6-mAb induces cell death, we performed a time- and concentration-dependent experiment using the...

In primary murine CD4 + T cells and human Jurkat T cells, CD47 functions as a negative regulator of the VEGF-VEGFR2 pathway at the level of receptor phosphorylation and downstream phosphorylation of Src. Binding of TSP1 to CD47 further inhibits VEGFR2 signaling in these cells.

2023年1月30日 · Elevated expression of CD47 in some cancers is associated with poor survival related to its function as an innate immune checkpoint when expressed on tumor cells. In contrast, elevated CD47 expression in cutaneous melanomas is associated with improved survival.

2013年11月1日 · As a control, we created stably transfected CD47 − Jurkat cells expressing full-length CD47 tagged with green fluorescent protein (GFP; CD47+GFP) or GFP alone (CD47 − GFPcont). The level of CD47 expression was similar to that for the parental clone (data not shown) and restored arrest to ICAM-1 to a level similar to that for the parent ...

CD47，称为整合素相关蛋白（IAP），是在人体中由CD47基因编码的跨膜蛋白，属于免疫球蛋白超家族，可以与整合素（Integrin）、血小板反应蛋白-1（TSP-1）和信号调节蛋白α（Sirpα）相互作用。