2024年10月1日 · The pathogenic Escherichia coli K4 strain produces a capsular fructosylated chondroitin, called K4 polysaccharide, synthesized by chondroitin synthase KfoC, which has been characterized and expressed in several microorganisms for the production of recombinant chondroitin [[3], [4], [5]].

2015年1月1日 · This study proposes a safer and more feasible approach to metabolically engineer chondroitin production by expressing genes from the pathogenic E. coli K4 strain, which natively produces a capsular polysaccharide that shares the similar structure with chondroitin, into the non-pathogenic E. coli BL21 Star™ (DE3) strain. The ePathBrick vectors ...

2023年10月1日 · Recent literature demonstrates that intracellular quantification of precursor UDP-sugars, that are necessary for fructosylated chondroitin biosynthesis in wild type and genetically engineered E. coli K4 strains, greatly varies according to the type of cultivation system (shakeflask, bioreactor) and fermentation strategy used (Cimini et al ...

The K4 strain of Escherichia coli-produced chondroitin synthase K4CP is one such bifunctional glycosyltransferase that catalyzes β1–3 glucuronyltransfer and β1–4-N-acetylgalactosaminlytransfer reactions to polymerize glucuronic acid (GlcA) and N-acteylgalactosamine (GalNAc) into a chondroitin chain [GlcA β(1–3)-GalNAc β(1–4)] n ...

2017年11月20日 · E. coli O5:K4:H4 (ATCC 23502), also known as K4, is a uropathogenic strain, and its whole genome has been sequenced (Cress et al. 2013a). Its capsular polysaccharide, commonly referred to as K4CP, is classified into the E. coli group 2 capsules (Whitfield 2006).

Escherichia coli strain K4 produces the K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone (GlcUA β(1–3)-GalNAc β(1–4)) n to which β-fructose is linked at position C-3 of the GlcUA residue. We molecularly cloned region 2 of the K4 capsular gene cluster essential for biosynthesis of the polysaccharide, and we ...

2014年10月31日 · We report the 4.682-Mbp high-quality draft assembly of the Escherichia coli strain ATCC #23502 (serovar O5:K4(L):H4, also known as NCDC U1-41) genome. This uropathogenic strain is produces a glycosaminoglycan-like capsular polysaccharide with a backbone similar in structure to unsulfated chondroitin, a precursor to the nutraceutically and ...

2022年7月10日 · Multiple bacterial strains have been used to make different types of chondroitin sulfate with varying yields. The most common strain used is E. coli K4 because it already contains the required chondroitin backbone. Both shake flasks and fermentation methods have been used to synthesize unsulfated chondroitin in E. coli K4 strain [61,62,63].

2020年8月6日 · In this work, we took advantage of recombinant E. coli K4 strains that overproduce capsular polysaccharide, to study whether the inferred pathway modifications also influenced the size of the...

2017年2月28日 · Escherichia coli K4 and K5 capsular polysaccharides (K4 and K5 CPSs) have been used as starting material for the biotechnological production of chondroitin sulfate (CS) and heparin (HP) respectively. The CPS covers the outer cell wall but in late exponential or stationary growth phase it is released in the surrounding medium.