Clonal HLA-A*0201-expressing K562 (K562/A*0201) cells were able to process and present endogenously expressed and exogenously loaded melanoma peptide antigens to HLA-A*0201-restricted cytolytic T lymphocyte clones in cytotoxicity and IFN-gamma ELISPOT assays.

K562 cells were the first human immortalised myelogenous leukemia cell line to be established. K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis .

We set out to generate a library of K562 cells transduced with common MHC class I and II antigens and costimulatory molecules for use as aAPC. We identified an inherent antiproliferative property of both wildtype and transduced K562 lines, which could be eliminated by fixation in …

2014年1月1日 · Fixed gene-modified K562 can serve as effective aAPC to expand CMV-specific cytotoxic T lymphocytes for therapeutic use in patients after stem cell transplantation. Our findings have implications for broader understanding of the immune evasion mechanisms used by leukemia and other tumors.

为了寻找用于ELISPOT测定的替代APC，用HLA-A * 0201基因转染了本身在细胞表面上不表达I类和II类HLA分子的人慢性骨髓性白血病细胞系K562。 表达HLA-A * 0201的克隆K562（K562 / A * 0201）细胞能够处理并向细胞毒性和IFN-γELISPOT中呈递内源表达和外源负载的黑素瘤肽抗原至HLA-A * 0201限制的溶细胞性T淋巴细胞克隆分析。 然后将K562 / A * 0201细胞用作IFN-γ斑点检测中的APC，以检测对源自巨细胞病毒，爱泼斯坦-巴尔病毒，流感病毒的已知HLA-A * 0201结 …

Clonal HLA-A*0201-expressing K562 (K562/A*0201) cells were able to process and present endogenously expressed and exogenously loaded melanoma peptide antigens to HLA-A*0201-restricted cytolytic T lymphocyte clones in cytotoxicity and IFN-γ ELISPOT assays.

人类细胞周期蛋白cyclin A2是细胞周期蛋白家族中重要的一员,参与Gl/S期和G2/M期调控,在DNA复制,转录和肿瘤转化和发展过程中有非常重要的作用.cyclin A2在许多肿瘤中过表达,可以当作肿瘤的标记.其表达水平是肿瘤恶性程度,化疗敏感性和预后的指标,因此临床评价肿瘤 ...

结论：（1）两细胞株染色休均为超二倍体结构，具有复杂染色体核型异常； （2）K562/A02细胞株为Bcr-abl融合基因高表达细胞株； （3）两细胞株之Bcr-abl融合基因abl激酶区DNA序列均未见点突变，K562/A02细胞耐药与abl激酶区点突变无关系。

Here we identified novel human leucocyte antigen (HLA)-A*0201 (HLAA2)-restricted cytotoxic T lymphocyte (CTL) epitopes from a B cell specific molecule HLA-DOβ (DOB) as a potential target for MM. By DNA microarray analysis, the HLADOB expression in MM cells was significantly higher than that in normal plasma cells.

Results: We further generated K562-based MHC class I-negative aAPC line by zinc-finger nuclease mediated insertion of costimulatory molecules into the AAVS1 locus. This aAPC line could attenuate allogeneic immune responses but support robust antigen-independent and CD19 antigen-specific chimeric antigen receptor-T cell expansion in vitro .