FLAG标记的Anti-K63 TUBE是从细胞和组织提取物或体外合成混合物中有效分离和富集K63-多聚泛素的理想试剂。 其由多个泛素蛋白相互作用基序（UIM）组成，与其它链接类型相比，K63-TUBE对K63链的多聚泛素的亲和力会强上1000至10,000倍，这将是一种极度灵敏且经济的工具。

泛素（Ubiquitin，Ub）是一种由76个氨基酸组成的小分子蛋白质，分子量约8.5kDa，在进化过程中高度保守，全长包含7个赖氨酸（Lys）位点（K6，K11，K27，K33，K48和K63）、一个位于N端的甲硫氨酸（Met）位点（M1）以及一个位于C端的甘氨酸（Gly）位点（G76）。

2024年11月25日 · Here, we report the discovery that USP53 and USP54 are active DUBs with high specificity for K63-linked polyubiquitin. We demonstrate how USP53 mutations abrogate catalytic activity, implicating...

2018年1月29日 · K63 ubiquitin chains facilitate subsequent assembly of K48/K63 branched ubiquitin chains through recruitment of ubiquitin ligases assembling K48 chains, thereby directing substrates to the proteasome. These results reveal an unappreciated ubiquitin-dependent pathway leading to the proteasome.

泛素ub修饰共有7种类型：k63，k48，k33，k29，k27，k11和k6，最常见是k48修饰（蛋白酶体降解）和k63修饰（其它生物学过程）。为了确定由hectd3介导的traf3多聚泛素化类型，构建不同ub突变体进行co-ip实验，结果发…

2022年5月2日 · The second most common type of polyubiquitin chain is the K63-linked polyubiquitin, which strongly connects to some self-degradative mechanisms in the cells. In this review, we discuss the role of this type of polyubiquitin pattern in …

2021年3月9日 · K63不突变的ubi，只有K63位的lys不突变，而其它位置的lys都发生了突变。 反之，K63突变的ubi（K63R）就是只有K63位点发生了突变，别的位置不突变（K63R见上图）。

2021年1月5日 · Therefore, K33- is the latest addition to the Ub linkages, mainly K63-, which may serve as protein sorting signals. Emerging in vivo imaging methods revealed the co-localization of K33-linked polyUb chains and Ub-binding autophagy adaptors, such as LC3B and its interacting partner SQSTM1 .

2020年12月18日 · We found that the presence of K63-linked ubiquitin chains on mitochondria resulted in the recruitment of the ubiquitin adaptor p62 and induced a dramatic redistribution of mitochondria, which was...

2020年1月10日 · Polyubiquitination, the sequential attachment of the small 8 kDa globular protein ubiquitin (Ub) to target proteins, represents a major posttranslational modification that ultimately determines the substrate’s cellular fate.