In chemistry, biochemistry, and pharmacology, a dissociation constant (KD) is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its component ions.

2019年2月2日 · Two commonly encountered parameters in enzyme kinetics are the Michaelis constant (Km) and the dissociation constant (Kd), both report aspects of a substrate’s binding behavior. In this article, I clarify these terms' definitions, differences and biochemical significance .

2017年10月7日 · 抑制常数（inhibition constant），反映的是抑制剂对靶标的抑制强度，这个值越小说明抑制能力越强， 某些情况下 可以与后文的Kd等同。 Ki为50%的酶E被抑制剂I结合时对应的游离抑制剂的浓度。 3） Kd. 解离常数 （dissociation constant），反映的是化合物对靶标的亲和力大小，值越小亲和力越强。 从此公式得出:Kd为50%的酶E被抑制剂I’结合时对应的游离抑制剂的浓度。 4） Ka. 结合常数 （association constant），与Kd相反，值越大亲和力越强。 5） Km. …

Kd, or the dissociation constant, reveals the affinity between an enzyme and its inhibitor, whereas Km, the Michaelis constant, measures the enzyme’s affinity for its substrate during catalysis. Grasping the nuances between Kd and Km will not only boost your knowledge but also sharpen your research and application skills in the lab.

2019年12月31日 · Ki refers to inhibition constant, while Kd means dissociation constant. Both terms are used to describe the binding affinity that a small molecule or macromolecule has for an enzyme or receptor. The difference is that Kd is a more general, all-encompassing term.

1/Ks是反映酶与底物亲和力的参数。因为当k2极小时Km就近似等于Ks，所以说1/Km可近似表示酶与底物的亲和力，1/Km越大，亲和力越大。解离常数在研究两个分子结合的时候经常写作Kd。 2. 酶的 转化数 (TN)与 催化常数 （kcat）

2024年10月1日 · K d is the dissociation constant, indicating the concentration of enzyme at which 50% of the DNA is bound. On the left side, [E], [S], and [ES] are the concentrations of free enzyme, free substrate, and the enzyme-substrate complex, respectively. Fraction bound (Equation 5) is calculated by substituting [ES] of Equation 4 into Equation 2.

2022年4月17日 · 抑制常数（inhibition constant），反映的是抑制剂对靶标的抑制强度，这个值越小说明抑制能力越强， 某些情况下 可以与后文的Kd等同。 K i 为50%的酶E被抑制剂I结合时对应的游离抑制剂的浓度。

2017年6月11日 · Kd is the dissociation constant. It tells you how well a specific substrate will bind to the enzyme. The smaller the Kd, the stronger the Substrate is bound to the Enzyme. Kcat is the rate of reaction of the enzyme; turnover of substrate to product / unit time. I should probably mention, you most likely will not see any Kcat questions on the MCAT.

Km stands for the Michaelis constant, a term you’ll encounter when delving into enzyme kinetics. It’s a measure that tells you about the enzyme’s efficiency. On the flip side, Kd, or the dissociation constant, is key in the realm of biochemistry, offering insights into the strength of the interaction between a ligand and its receptor.