Using KLRG1 knockout mice, we found that KLRG1 deficiency does not affect Treg cell frequencies in spleen, mesenteric lymph nodes or intestine, or frequencies of GATA3 + Treg cells in the gut. KLRG1-deficient Treg cells were also protective in a T-cell transfer model of colitis.

Studies in mice suggested that the expression of this gene may be regulated by MHC class I molecules. [9] KLRG1 is a lymphocyte co-inhibitory, or immune checkpoint, receptor expressed predominantly on late-differentiated effector and effector memory CD8+ T and NK cells.

Here, we show that LLEC from mice express a distinct phenotypic and transcriptional signature that shares characteristics of both early effectors and long-lived memory cells. We also find that in contrast to KLRG1+ effector cells, LLEC undergo homeostatic proliferation and are not critically dependent on IL-15 for their maintenance.

To investigate the role of plasticity in effector-to-memory CD8 + T cell differentiation and its impact on memory CD8 + T cell heterogeneity and recall responses, we generated a mouse strain that expressed an eGFP-Cre recombinase (Cre) fusion protein under the control of the Klrg1 gene (Klrg1 Cre) (Figure S1A).

2021年5月1日 · Furthermore, α-KLRG1 antibody treatment in lung tumor bearing KP mice showed prolonged survival, accompanied by reduced proportion of KLRG1+ Tregs in the tumor. Finally, immunogenomic characterization of KLRG1 KO/wt and tumor infiltrating KLRG1+/− Tregs revealed distinct gene expression patterns and associated immune pathways.

2017年4月24日 · Using KLRG1 knockout mice, we found that KLRG1 deficiency does not affect Treg cell frequencies in spleen, mesenteric lymph nodes or intestine, or frequencies of GATA3 + Treg cells in the gut. KLRG1-deficient Treg cells were also protective in a …

3 天之前 · To investigate the role of Atf4-expressing T regs in the obese liver, we generated ATF4-inducible conditional KO mice (Atf4 fl/fl Foxp3 eGFP-Cre-ERT2 mice or Atf4iKO mice) ... Furthermore, lean Atf4iKO mice had decreased KLRG1 + cells, CD49b + cells, and PD-1 + KLRG1 + cells in the spleen compared with lean control mice (fig. S10, E and F).

3 天之前 · In the spleens of QRICH1 T cell KO mice, ... Compared with control cells, an increased percentage of QRICH1-KO OT-1 CD8 + T cells was KLRG1 + terminal effector cells, and a decreased percentage was CD127 + memory precursor cells (Fig. 7, D and E). We observed a similar trend for KLRG1 expression in the blood at day 5 postinfection, whereas ...

2017年5月1日 · KLRG1 is an inhibitory receptor of T cell function and its expression is elevated on T cells with increasing age and T cell differentiation status. We have previously shown using KLRG1-KO mice that the absence of KLRG1 provides a survival advantage to chronic M.tb infected mice, showing proof of concept for an inhibitory role of this receptor ...

2016年1月1日 · We observed that, during per-oral microsporidial infection, IL-21 was critical for the generation of an optimal effector CD8 T cell immunity. Sharply decreased effector KLRG1 + CD8 response was observed in IL-21R knockout mice, and although these cells exhibited reduced functional properties, they retained the ability to proliferate. The role ...