What is the meaning behind Kcat / Km? - Biology Stack Exchange
2016年11月19日 · $\begingroup$ "There is an upper limit to Kcat / Km, imposed by the rate at which E and S can diffuse together in an aqueous solution. This diffusion controlled limit is …
biochemistry - kcat in uncompetitive enzyme inhibition - Biology …
2020年7月30日 · Aside from grammar and punctuation fixes, your question needs attention to the following issues: 1. Please mention the source of the information (kcat remaining unchanged …
biochemistry - Michaelis-Menten equation; how to find the …
I was wondering what the constants in the Michaelis-Menten equation actually mean in experimental data of enzymes. How do I process the data to find Km and Kcat? I did an …
How to calculate the turnover number? - Biology Stack Exchange
2018年11月17日 · However the formula I have been given is Kcat = specific activity/molecular mass of enzyme. What is the relationship between these two different formulas - why do they …
Is there any way to estimate the kcat of an enzyme if we don't …
2025年1月31日 · I thought that perhaps one could fit the Michaelis-Menten equation to a series of progress curves at different substrate levels, but then I realized the fit wouldn't be able to …
In what cases can $k_{\\rm cat}/K_{\\rm M}$ be used to compare ...
2024年5月29日 · Stack Exchange Network. Stack Exchange network consists of 183 Q&A communities including Stack Overflow, the largest, most trusted online community for …
Enzyme kinetics: Effect of immobilization on kinetic parameters
2016年3月21日 · kcat 0.5 1/min Km 0.6 microM [E0] 5 micro M [S] 60 micro M Could I expect to retain them after immobilization? Or are these too high for an immobilized enzyme? If it …
Effect of pH, temperature and enzyme concentration on Km
2022年1月2日 · Another issue which makes me think so: In all the biochemistry literature that I have encountered Km and Kcat appears for each enzyme with no indication of the …
Why does uncompetitive inhibition decrease the Michaelis constant?
2016年6月27日 · kcat in uncompetitive enzyme inhibition. Hot Network Questions Does training a neural network on a ...
Does pH affect Michaelis constant? - Biology Stack Exchange
2015年8月22日 · $\begingroup$ @wswr kr is dissociation rate constant. kcat is the turnover number or the rate constant of product formation. Both these reduce the concentration of the …