Kcat/Km就可以用来确定酶的最适底物，同一个酶对不同底物的Kcat/Km最大可相差100万倍。 当利用蛋白质工程对酶进行改造时，不同突变型的Kcat/Km也是需要测定的参数，用来表征酶催化效率的变化情况。

For this, we define the value Kcat , also known as the turnover number. Mathematically, To determine Kcat, one must obviously know the V max at a particular concentration of enzyme, but the beauty of the term is that it is a measure of velocity independent of enzyme concentration, thanks to the term in the denominator.

Kcat is the turnover number -- the number of substrate molecule each enzyme site converts to product per unit time. If you know the concentration of enzyme sites, you can fit Kcat instead of Vmax when analyzing a substrate vs. velocity curve.

2024年9月22日 · 酶催化效率（kcat）是生物化学和酶工程领域中的一个关键参数，用于描述酶在单位时间内催化底物转化为产物的能力。 enzyme_kcat数据集由SysBioChalmers团队于2022年创建，基于DLKcat项目，整合了BRENDA和SABIO-RK数据库中的酶催化效率数据，并通过Biomap平台进行了数据清洗和分割。 该数据集的核心研究问题在于通过机器学习模型预测酶的催化效率，从而加速酶工程和生物催化剂的开发。 其影响力体现在为酶催化效率的定量预测提 …

TABLE 6-8 Enzyme 10 5 10 2 10 2 10 5 10 5 Enzymes for Which kcat/Km Is Close to the Diffusion kcat -Controlled Limit (10 (M) 1.2 x 2.6 x 8 to 109 M S kcat /Km Substrate Acetylcholine

When calculating K cat, the concentration units cancel out, so K cat is expressed in units of inverse time. It is the turnover number -- the number of substrate molecule each enzyme site converts to product per unit time. Fitting Kcat with Prism. You can also determine the K cat directly by fittng this model to your data.

Calculate the Turnover Number (kcat) for enzyme kinetics analysis. Enter Vmax and total enzyme concentration to calculate kcat. The Turnover Number (kcat) is a key parameter in enzyme kinetics that represents the maximum number of substrate molecules converted to product per enzyme molecule per second.

2024年10月6日 · Kcat定义为在最理想条件下，酶催化生成底物的速率，即单位时间内酶分子转化底物的数量。 Kcat的单位为1/s，表示单个酶分子每秒转化底物的数量。

Km俗称米氏常数，以浓度做单位，米氏常数定义为反应速度达到最大反应速度一半时的底物浓度。 Km可以反映出酶与底物的亲和力，Km越低，亲和力越大。 kcat/Km称为催化效率，常常以此来比较不同的酶而同一底物，或者不同底物而同一种酶。

How to Calculate the Turnover Number (Kcat) of Enzymes? K cat is a parameter that measures the catalytic efficiency of an enzyme. The K cat value represents the number of substrate …