Kcat/Km就可以用来确定酶的最适底物，同一个酶对不同底物的Kcat/Km最大可相差100万倍。 当利用蛋白质工程对酶进行改造时，不同突变型的Kcat/Km也是需要测定的参数，用来表征酶催化效率的变化情况。

In chemistry, the term " turnover number " has two distinct meanings. In enzymology, the turnover number (kcat) is defined as the limiting number of chemical conversions of substrate molecules per second that a single active site will execute for a given enzyme concentration [ET] for enzymes with two or more active sites. [1] .

For this, we define the value Kcat , also known as the turnover number. Mathematically, To determine Kcat, one must obviously know the V max at a particular concentration of enzyme, but the beauty of the term is that it is a measure of velocity independent of enzyme concentration, thanks to the term in the denominator.

kcat/Km称为酶的专一性常数，它不受非生产性结合与中间产物积累的影响，可以表示酶对相互竞争的几种底物的专一性。 生理条件下许多反应的底物浓度是很低的。

Calculate the Turnover Number (kcat) for enzyme kinetics analysis. Enter Vmax and total enzyme concentration to calculate kcat. The Turnover Number (kcat) is a key parameter in enzyme kinetics that represents the maximum number of substrate molecules converted to product per enzyme molecule per second.

K M is a dependent variable, and its value can change due to many reasons, including the pH level of the system, temperature, or any other condition that might affect a chemical reaction. A small K M indicates that the substrate has a high affinity for the enzyme.

Kcat, or catalytic constant, is the number of maximum substrate molecules converted by the enzyme molecule into a product per unit time at the saturation of substrate on the enzyme.

Kcat is turnover number, expresed as number of substrate molecules turned into product per enzyme site per minute. Km and X are both in the same units of substrate concentration.

2022年6月13日 · kcat / KM is a fundamental kinetic parameter as important as kcat in enzyme kinetics. Although the parameter provides critical information on how the enzyme lowers the energy barriers along the reaction pathway for catalysis, kcat / KM is frequently considered as a composite or derived parameter to assess both kcat and KM together rather than ...

The K cat value represents the number of substrate molecules an enzyme can convert into a product per unit of time when fully saturated with substrates. K cat is also referred to as the …