RNA-binding protein kinetics - Nature Methods
2021年2月10日 · The key to KIN-CLIP is fast RNA–protein crosslinking, relative to association and dissociation rate constants for the RNA–protein interaction, and the researchers use a …
The kinetic landscape of an RNA-binding protein in cells
2021年2月10日 · This kinetic cross-linking and immunoprecipitation (KIN-CLIP) approach reveals that DAZL resides at individual binding sites for time periods of only seconds or...
细胞中 RNA 结合蛋白的动力学景观,Nature - X-MOL
这种动力学交联和免疫沉淀 (kin-clip) 方法揭示了 dazl 在单个结合位点的停留时间仅为几秒或更短，而结合位点保持无 dazl 的时间明显更长。 数据还表明 DAZL 与多个近端位点簇中的许多 …
The kinetic landscape of an RNA-binding protein in cells
This kinetic cross-linking and immunoprecipitation (KIN-CLIP) approach reveals that DAZL resides at individual binding sites for time periods of only seconds or shorter, whereas the binding …
Kinetics of RNA–protein interactions in cells - Cell Press
A new method, kinetic crosslinking and immunoprecipitation (KIN-CLIP) now allows the determination of RBP association and dissociation rate constants at thousands of individual …
RNA-binding protein kinetics,Nature Methods - X-MOL
KIN-CLIP allows determination of binding and dissociation kinetics of RNA-binding proteins in cells.
GitHub - deebratforlife/KIN-CLIP: Scripts for KIN-CLIP …
Scripts for KIN-CLIP manuscript. More details can be provided upon request. Resources
A New Laser-based Approach to Measure RNA-protein Interactions within ...
2021年5月24日 · The KIN-CLIP approach uses a femtosecond (fs) ultraviolet (UV) laser that efficiently increases the RNA-protein crosslinking without altering their crosslinking patterns. …
Dynamics of RNA–protein interactions studied in living cells - Nature
2021年2月10日 · Sharma et al. 2 describe a method called KIN-CLIP that uses ultrafast pulses of ultraviolet light to generate covalent crosslinks between the bound proteins and RNA …
Rapidly Growing Protein-Centric Technologies to Extensively
2021年4月20日 · Recently, a pulsed femtosecond UV to CLIP methodology (kinetic cross-linking and immunoprecipitation; KIN-CLIP) has been reported . Compared with conventional UV …