2012年3月1日 · Measurements of kLa provide important information about a bioprocess or bioreactor. These determinations ensure that processing conditions are such that an adequate supply of oxygen is available for proliferation of cells. The kLa value can also be used to optimize control variables over the lifecycle of a bioprocess.

2020年3月15日 · Significant research efforts have been expended over the last decades in order to develop reliable and easy to apply methodologies for determining kL a using aeration experiment and fast oxygen (polarographic) probes.

We evaluated the sparger kLa and kLaCO2 (CO 2 volumetric mass transfer coefficient) from a range of bioreactor scales (3-2,000 L) with different spargers. Results demonstrated that kLa for oxygen is not an issue when scaling from small-scale to large-scale bioreactors at the same gas flow rate per reactor volume (vvm).

2021年10月24日 · The OTR is defined by the kLa and the difference between the oxygen concentration of the introduced gas and the oxygen concentration in the medium:

2013年12月2日 · Since there is no known universal correlation between the volumetric mass transfer coefficient for oxygen k L a and relevant operating parameters in such bioreactor systems, the aim of this current study is to experimentally determine a universal k L a correlation.

2010年1月1日 · A dynamic method of measuring the volumetric mass transfer coefficient of oxygen, kLa, in gas-liquid reactors is presented which consists in monitoring simultaneously the pressure and...

2013年5月15日 · In this study, it was clarified that a high kLa of 300 h⁻¹ or higher can be measured by a static method using an optical dissolved oxygen sensor with a remarkably fast response time.

In this study, we quantify the volumetric mass transfer coefficient of oxygen (kLa) and specific power input (P/V) in bioreactors. These are essential parameters that define a bioreactor's operating conditions and are used to address bioprocess development challenges, namely, developing scale-up/scale-down models and tech-transfer.

In this study, a 15 L mammalian cell culture bioreactor was characterized with respect to k L a under varying process conditions. The resulting dynamic k L a description combined with functions for the calculation of oxygen concentrations under prevailing process conditions led to an easy-to-apply model, that allows real-time calculation of the ...

2020年3月1日 · 在过去的几十年中，为了开发可靠且易于应用的方法来使用曝气实验和快速氧气（极谱）探针确定 kLa，已经进行了大量的研究工作。 本研究对这些方法进行了审查，然后介绍了一种新的、有前途的 kLa 测定方法，可在无法获得分步实验数据时使用。