Bubble size distribution (BSD), gas holdup, and kLa were determined for different types of liquids, relevant fermentation conditions and superficial gas velocities uG.

2012年3月1日 · Measurements of kLa provide important information about a bioprocess or bioreactor. These determinations ensure that processing conditions are such that an adequate …

Since there is no known universal correlation between the volumetric mass transfer coefficient for oxygen k L a and relevant operating parameters in such bioreactor systems, the aim of this …

In aerobic bioprocess applications, kLa is a critical parameter for the evaluation of the efficiency of a bioreactor in terms of how effectively oxygen is supplied to the cultured organism. This short …

This study is focused on the evaluation of the volumetric mass transfer coefficient (k L a) of E. coli fermentation producing β-glucuronidase enzyme in different bioreactor operating conditions.

Table 3. KLa determination using the data reported in Biard et al. [1]. For all the simulations: gas flow-rate = 1.20 m3/s (gas EBRT = 2 s); liquid flow-rate = 3.06 × 10−3 m3/s; and V = 2.36 m3 …

dard operating procedure (SOP) for kLa measurements. Using this protocol, diferent manufacturers experimentally determine. the kLa values of single-use bioreactors they ofer. A …

In choosing a simulation resolution, we consider three intrinsic eddy length scales: the integral scale, the Taylor scale, and the Kolmogorov scale. The integral length scale, l, which …

Single-use bioreactors are increasingly used in research, process development, and production. Like conventional reusable systems they have to allow the setup of optimal growth conditions.

Table of Contents. 1. Chemical Method: 2. Dynamic Differential Gassing-Out (DDGO) Method: 3. Dynamic Integral Gassing-Out (DIGO) Method: 4. Oxygen Balance (OB) Method: 5. Enzymic …