Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21 (DE3)-derived strains.

We have developed a new E. coli host, KRX, that provides protein yields comparable to those of BL21(DE3) but with much higher transformation effi-ciencies. This new KRX strain allows cloning and protein overexpression using a single strain.

2017年9月28日 · Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications.

ase rhamnose-inducible system. Description The Single-Step (KRX) Competent Cells are an E. coli K strain that is designed for both efficient transformation (>108 cfu/μg) and tightly-controlled protein expression. The stringent control provided by …

Single Step (KRX) Competent Cells are prepared from the KRX strain, an E. coli K12 derivative with salient features associated with cloning and screening strains plus engineered attributes to optimize controlled protein expression.

2017年9月28日 · Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications.

Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications.

The draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications and offers both a high transformation efficiency and the possibility to produce the target protein to high yields in …

2010年11月12日 · KRX protein expression strain (Promega) is a T7 expression strain where the T7 RNA polymerase is controlled by the PrhaBAD promoter. This is a relatively new strain and we expect it will perform well for the controlled expression of toxic proteins.

Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21 (DE3)-derived strains.