We established simultaneous dual color two-photon microscopy to visualize the trafficking of Neuropeptide Y (NPY)-, LAMP1-, and RAB7-tagged organelles in thalamocortical axons …

(H) Kymograph of mEmerald tag and lysosomes in axons. Rat cortical neurons were transduced with LAMP1-HaloTag to label lysosomes and PGK promoter driven mEmerald tag. Time-lapse …

2024年12月4日 · 用于固定样本中溶酶体的免疫荧光染色的标记物包括但不限于以下几种： LAMP1（Lysosome-associated membrane protein 1）：溶酶体相关膜蛋白1，是溶酶体膜的主 …

2019年6月27日 · Kymograph analysis of LAMP1‐mCherry‐labeled lysosomes showed that upon overexpression of myoV DN, lysosome motility increased, as judged by the decrease in time …

2022年12月2日 · In this study, we utilized dual color two-photon imaging to analyze the trafficking dynamics of three organelle markers: the DCV marker Neuropeptide Y (NPY), and the endo …

LAMP1-EGFP knock-in iPSCs and i 3 Neurons as a tool to study intracellular transport of endogenously-tagged vesicles. Most studies investigating axonal or dendritic transport rely on …

LAMP1 and RAB7 in thalamocortical axons in intact living brain. In addition, to gain insight into local DCV slow down at synapses, we combined DCV imaging with an axonal-targeted …

2024年6月3日 · The KymoButler software 70 uses artificial intelligence to trace the lines in a kymograph (input: left, bright images, output: right, dark images) and extracts information …

In BORCS7-KO, ARL8A-B-KO, or KIF1B-5B-KO HeLa cells, LAMP1/CD63-positive endolysosomes became even more concentrated in the juxtanuclear area, with concomitant …

2016年10月10日 · Kymograph axes are the same as in Figure 1. (F) Representative kymograph of mitochondria (red) and LAMP1 (green) in the axon of a cultured cortical neuron at 28 DIV. …