2025年1月29日 · The role of autophagy in cisplatin anticancer action was investigated using human U251 glioma, rat C6 glioma and mouse L929 fibrosarcoma cell lines. A dose- and time-dependent induction of autophagy was observed in tumour cells following cisplatin ...

2021年3月28日 · TNF treatment induces activation of AMPK. (A) L929 cells were transfected with non-targeting (siCtrl) or Ripk3 siRNAs (siRipk3). 48 h post transfection, cells were exposed to 10 ng/ml TNF and 30 µM QVD (TQ) for the indicated times. Then, whole cell lysates were subjected to SDS-PAGE and immunoblotting for indicated proteins.

2021年8月10日 · Phosphorylation of S79 in ACC (acetyl-CoA carboxylase) by AMPK is a biomarker for cellular starvation response 52.

2020年2月27日 · The L929 cell viability was affected by the concentration increment and was dropped by 20% and more than 50% after treatments with 20 and 40 μM Compound C for 24 h, respectively. The inhibitory effect of Compound C on AMPK activities was also tested by examining the AMPK phosphorylation levels.

2015年5月22日 · We demonstrate herein that intracellular nicotinamide adenine dinucleotide (NAD +) has an important role in supporting cell progression to necroptosis. Using a panel of pharmacological and genetic...

2021年3月15日 · AMPK agonists increase the level of Ak4 and silencing AMPKα1/2 reduces Ak4 expression (Figure 4B; Supplementary Figure 5A). Based on our results together with published data, we propose that Ak4 through promoting Hif1a transcription and inhibiting AMPK activation positively regulates inflammation gene expression.

ampk 属于 高度保守的真核蛋白质，由 α、β、γ 三个亚基按 照 1:1:1 的比例组成，是一种多亚基蛋白。 AMPK 的 α 亚基为催化亚基，对蛋白激酶复合物的活性 起决定作用，而调节亚基 β 和 γ 只在一定程度上 参与该过程[4-5]。

In this study, we report that in L929 cells glucose uptake increases 6-fold as the pH of the uptake solution is increased from 6 to 9 with the half-maximal activation at pH 7.5; consistent with the pKa of cysteine residues.

探索腺苷酸活化蛋白激酶 (AMPK)与 cGAS-STING 通路之间的联系及其在先天免疫中扮演的角色。 利用 CRISPR/Cas9 技术、蛋白质印迹、RT-qPCR 等方法，探究 AMPK 对 DNA 相关免疫通路的调控机制。 在 HT-DNA 和 cGAMP 刺激下，AMPK-/-细胞株的 IFN-β 的表达量明显高于野生型细胞株，但这种变化在 RNA 信号通路中并不明显；激活 AMPK 可以抑制细胞内的 DNA 信号通路；在 DNA 信号通路中，AMPK-/-细胞株相较于野生型细胞株，STING 在 RNA 和蛋白水平上都明显 …

2016年3月31日 · TNFα-induced cytotoxicity in L929 cells was inhibited by gelatin-coating culture. The cells on gelatin-coated dishes showed reduced cellular ATP levels and increased adenosine 5′-monophosphate (AMP)–activated protein kinase (AMPK) phosphorylation, leading to increased ROS generation and autophagy.