所以说，BMDM细胞养的好不好，L929细胞的质量是关键。 为什么用L929可以刺激单核细胞分化为巨噬细胞呢，这就是之前说的，L929细胞它也是一种巨噬细胞集落刺激因子嘛。 我们之前也有用 DMEM培养基 试过，其实和1640培养基差不多，重点还是L929。 看似很简单的步骤，但是这个细胞可不是那么好养哦。 一来呢，他是原代细胞，没那么好，二来呢，L929能不能养好还不知道呢。 BMDM小鼠骨髓源性细胞培养 提取完成的单核细胞在10cm中皿培养过夜（提取步骤在前 …

在l929细胞条件培养基培养体系中，bmdm增殖能力显著高于pm (p < 0.001)。 吞噬实验发现，基础状态下BMDM吞噬能力显著高于PM ( P < 0.01)，经LPS刺激24 h后，除低剂量LPS (0.1 μg/mL)外，BMDM吞噬能力均显著高于PM ( P < 0.01或 P < 0.001)，提示BMDM吞噬能力在基础和激活状态下均强 ...

2021年4月14日 · BMDMs are a key model system to study macrophage biology in vitro. Commonly used methods to differentiate macrophages from BM are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 cell-conditioned media (LCCM) and how it affects the BMDM phenotype.

2019年1月29日 · 我已经改用l929上清液啦（30%过滤后l929上清液，20%血清，1%双抗，余DMEM） 在骨髓细胞冲到细胞皿，重悬后加1-3ml红细胞裂解液，等比例加入中和培养基，重悬后去上清，换最终用的培养基，过细胞筛，可以培养咯！

Here, we describe a protocol for the isolation of mouse bone marrow cells and subsequent macrophage differentiation in which bone marrow cells are cultured in the presence of M-CSF, supplemented either by conditioned medium from L929 cells or in purified form.

l929培养 • Organism: Mus musculus, mouse • Morphology: fibroblast • Medium: complete DMEM(10% FBS) • Subculturing: 2XTE, 1 min • 100 mm dish, 10 million

We describe a new and optimised protocol to culture BMDMs. We used classically activated macrophages isolated from WT and NO-deficient mice. This study emphasises the importance of measuring culture media components and their effects. GMCSF priming enhances NO secretion upon inflammatory stimulation.

摘要 [目的]通过比较常用的m-csf与l929细胞培养上清两种诱导方式获得骨髓巨噬细胞(bmdm)的形态及基本生物学功能,为选择制备bmdm的方法提供实验依据。[方法]用两种方式诱导小鼠骨髓获取bmdm,然后分别利用zeis...

2021年6月1日 · BMDMs are a key model system to study macrophage biology in vitro. Commonly used methods to differentiate macrophages from BM are treatment with either recombinant M-CSF or the supernatant of...

In this protocol, bone marrow cells are grown in culture dishes in the presence of M-CSF, which is secreted by L929 cells and is used in the form of L929-conditioned medium. Under these conditions, the bone marrow monocyte/macrophage progenitors will proliferate and differentiate into a homogenous population of mature BMMs.