2014年11月17日 · Rip3 expression was efficiently decreased in L929 cells by RNAi, sufficient to prevent TNFα/zvad-induced necroptosis, but not RSL3-induced cell death , or Gpx4 deletion-induced ferroptosis in ...

2022年3月9日 · In this study we explored a novel mechanism by which the lipid peroxidation product HNE inhibits the NLRP3 inflammasome by directly disrupting the binding of NEK7 to NLRP3, thereby preventing...

2023年2月1日 · Here, we engineered a sequential pH/adenosine triphosphate (ATP)-sensitive nanomedicine by initially synthesizing zeolitic imidazolate framework 90 (ZIF90) containing metformin (Met) and RSL3 (Met/RSL3@ZIF90) and then immobilizing it into iron ions and tannic acid (TA) integrated nanoscale coordination polymer (Fe-TA NCP) to obtain Met/RSL3@ZIF9...

2023年6月1日 · Small molecules that trigger ferroptosis include RSL3, ML162, and ML210; these compounds are often used in studies of ferroptosis and are generally considered as GPX4 inhibitors. Here, we found that RSL3 and ML162 completely lack capacity of inhibiting the enzymatic activity of recombinant selenoprotein GPX4.

2024年5月27日 · We observed the ferroptosis of different subtypes of microglia under the conditions of ferroptosis inducer RSL3 and OGD/R. The results showed that RSL3 and OGD/R could cause significant ferroptosis in M2 microglia, while M1 microglia had a higher resistance.

Compounds 10, 33, 38, and 39 were able to inhibit tumor necrosis factor (TNF)-induced necroptosis in murine L929 cell lines. Functional experiments verified that compounds 10 and 39 inhibited necroptosis by downregulating the phosphorylation of RIPK3 and MLKL.

H9c2 cells were treated with 0.5 μM RLS3 in the presence of ferroptosis inhibitors (ferrostatin-1, Fer-1; liproxstatin-1, Lip-1), an apoptosis inhibitor (zVAD-fmk, zVAD), or a necroptosis inhibitor...

2019年11月4日 · RNA-seq and qRT-PCR analyses suggested that GPX4 downregulation occurred at the transcriptional level. Depletion or inhibition of GPX4 using specific siRNA or the chemical inhibitor RSL3,...

方法: 1.体外研究:以成纤维细胞L929为研究对象,首先通过CCK-8法检测RSL3(铁死亡激活剂)和NAC的细胞毒性并确定适宜的药物实验浓度.将细胞实验分为空白对照组(Control组),铁死亡模型组(RSL3组)和实验组(NAC组).通过细胞划痕实验观察在铁死亡状态下NAC对细胞增殖能力的 ...

2017年5月27日 · Like the murine fibroblastoma L929 cells, HT-29 cells express wildtype, rather than oncogenic HRAS, which renders them less sensitive to erastin-induced and RSL3-induced ferroptotic cell death .