LBA4404 (pSoup) 农杆菌感受态细胞经特殊工艺制备,经 pGs2质粒检测, 转化效率可达103cfu/μg,-70°C 保存12 个月转化效率不发生改变。 6000rpm 离心1 分钟收菌,留 100μl 左右上清,轻 轻吹打重悬菌体,取 适量菌液,涂 布于相应抗生素的LB 平板上,于 28°C培养箱中倒置培养48-72 小时。 ( 实验表明当平板只含有50μg/ml 的Kan 时,28°C 培养48 h 即可看到菌落;平 板中含有50 μg/ml Kan和20 μg/ml Rif 时,需 28°C 培养60 h 可看到菌落; 如果平板中含有50 μg/ml Kan 和50 μg/ml Rif 时则 …

ElectroMAX™ A. tumefaciens LBA4404 Cells are Agrobacterium cells that have been specially prepared for transformation by electroporation. ElectroMAX™ LBA4404 Cells contain the disarmed Ti plasmid pAL 4404, which has only the vir and ori region of the Ti plasmid.

碧云天生产的LBA4404根瘤农杆菌甘油菌(转基因植物构建用)，LBA4404 agrobacterium tumefaciens glycerol stock for construction of transgenic plant，是收集生长在对数期的LBA4404根瘤农杆菌，加入含有甘油的细菌

GoldBio’s LBA4404 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

LBA4404菌株为Ach5型背景，核基因中含有筛选标签----利福平抗性基因rif，为了便于转化操作， 此菌株携带一无自身转运功能的章鱼碱型Ti质粒pAL4404，此质粒含有vir基因(vir基因是T-DNA插入植

pAL4404型Ti质粒携带筛选标签Strep，赋予LBA4404菌株链霉素抗性，适用于菸草、番茄、烟草等植物的转基因操作。 我司提供的 LBA4404 化学转化感受态细胞经特殊工艺制作，基因型 Ach5 (rifR) Ti pAL4404 (strepR) Octopine ，经pCAMBIA2301质粒检测转化效率>104cfu/μg DNA。

LBA4404抗性及产品特点，培养基，LB media（可能出现LBA4404聚集)、SOC media、YM media（避免出现LBA4404聚集）

LBA4404菌株为农杆菌Ach5型背景(Hoekemaetal.,1983)，核基因中含有利福平抗性基因(Rif)。 此菌株还 携带一种自身转运功能丧失的质粒(disarmedTiplasmid)，LBA4404菌株含有章鱼碱型Ti质粒pAL4404，该质粒

GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.

Agrobacterium tumefaciens strain LBA4404以及binary vector method首先在荷兰的Leiden University被Dr. P. J. Hooykaas发现。 Agrobacterium tumefaciens含有pAL4404质粒，其只 …