在哺乳动物中，存在 4 种LC3异构体（LC3A、 LC3B 、 LC3B2和 LC3C），在不同的组织细胞中表达会有差异。 目前主要研究的是LC3A和LC3B，LC3C在许多细胞中表达量很低，研究的也 …

An increase in LC3-II band intensity and a decrease in LC3-I expression is considered the hallmark of autophagy but increases in LC3-II can be caused by enhanced autophagosome …

Microtubule-associated protein light chain 3 (LC3) is now widely used to monitor autophagy. One approach is to detect LC3 conversion (LC3-I to LC3-II) by immunoblot analysis because the …

在哺乳动物中，存在 4 种LC3 同工型（LC3A、LC3B、 LC3B2和 LC3C），在不同的组织细胞中表达会有差异。 目前主要研究的是LC3A和LC3B， LC3A 主要在 大脑 中表达，其他组织的表达 …

LC3-I 通过 Atg7 和 Atg3（分别对应 E1 和 E2 样酶）参与的泛素样反应，使 PtdEth（PE) 偶联，生成脂质化形式的 LC3，也称作 LC3-II，它可以附着到自噬体 (autophagosome) 的膜上，是自 …

Besides LC3-I/II bands, I see a signal above ~40kDa also in my samples. Is it a non-specific band or potentially a dimer of LC3? In literature, the LC3 homolog GABARAP has been shown to …

2019年10月31日 · When I tried to detect endogenous LC3-II in GFP-LC3 expressing cells, I would get very fain bands. if that is not your case, try running a time course and see if your cells …

In brains and neurons you'll see more LC3-I than -II and in HeLa you'll see the reverse. Sometimes you might only see the one band in those cells/tissue types. Use LC3 antibody …

LC3, however, is the only one known to form a stable association with the membrane of autophagosomes. It is known to exist in two forms: LC3-I, which is found in the cytoplasm, and …

Scientific answers to FAQs on LC3 and autophagy, including LC3 Western blot troubleshooting, the LC3-I/LC3-II ratio, and using autophagy markers and controls.