LC3-positive structures are prominent in autophagy-deficient cells
2019年7月12日 · However, while the existing literature shows the formation of LC3-positive structures upon GFP-LC3 overexpression in autophagy-null cells from mice (MEFs) 9, 10, 11, …
Autophagosomes in GFP-LC3 Transgenic Mice - PubMed
The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe …
Methods for Measuring Autophagy in Mice - PMC
A first double-transgenic mouse was generated by cross-breeding cardiac-specific mCherry-LC3 mice with systemic GFP-LC3 mice (αMyHC-mCherry/GFP-LC3 mice) to examine the role of …
027139 - CAG-RFP-EGFP-LC3 Strain Details
027139 C57BL/6-Tg (CAG-RFP/EGFP/Map1lc3b)1Hill/J <i>CAG-RFP-EGFP-LC3</i> transgenic mice express <i>RFP</i> and <i>EGFP</i> in a pH-dependent manner in phagocytic cells …
Autophagy-monitoring and autophagy-deficient mice - Taylor …
2017年1月30日 · Transgenic mice expressing GFP-LC3 in all tissues under the control of the constitutive Cag promoter have been widely used. 3 The presence of autophagosomes in …
Methods for monitoring autophagy using GFP-LC3 transgenic mice
Although electron microscopy has been the gold standard, recent studies of the molecular mechanism of autophagy have led to the development of several marker proteins for …
Transgenic expression of GFP-LC3 perturbs autophagy in exocrine ...
Transgenic GFP-LC3 mice are a valuable tool to investigate autophagy ; however, comparison of homeostatic and disease responses between GFP-LC3 and wild-type (WT) mice has not been …
Chapter 2 Methods for Monitoring Autophagy Using GFP‐LC3 Transgenic Mice
2009年1月1日 · Although electron microscopy has been the gold standard, recent studies of the molecular mechanism of autophagy have led to the development of several marker proteins …
Autophagosomes in GFP-LC3 Transgenic Mice | SpringerLink
The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe …
使用GFP-LC3转基因小鼠监测自噬的方法。,Methods in …
通过生成绿色荧光蛋白（GFP）-LC3转基因小鼠，该方法已应用于整个动物。 本章介绍了使用GFP-LC3转基因小鼠模型的这种方法的背景和实用性，以及在应用中的可能的预防措施。