LCLs are EBV-transformed lymphoblastoid B-cell lines, which can be easily established by EBV infection or derived spontaneously from peripheral blood B lymphocytes. You might find these chapters and articles relevant to this topic. Sarah E. Lee, ... Faycal Guedj.

2017年3月8日 · Lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV) serve as an unlimited resource of human genomic DNA. The protocol that is widely used to...

2021年5月25日 · Here, we extensively profiled a panel of human, chimpanzee, gorilla, orangutan, and macaque lymphoblastoid cell lines (LCLs), using ChIP-seq for five histone marks, ATAC-seq and RNA-seq, further...

2018年6月20日 · Lymphoblastoid cell lines (LCLs) have been by far the most prevalent cell type used to study the genetics underlying normal and disease-relevant human...

Infection of human B cells with EBV in vitro results in their immortalization and the resulting cell lines are named lymphoblastoid cell lines (LCLs) (1). In these cells, EBV establishes mainly a latent infection, characterized by the expression of a limited number of viral proteins.

2018年8月3日 · Whole blood is collected from research participants, and peripheral blood mononuclear cells are isolated and transformed into lymphoblastoid cell lines (LCLs) with Epstein Barr Virus. LCLs are grown in medium as growth plateaus due to …

Transformation of peripheral B lymphocytes by Epstein-Barr virus (EBV) is the method of choice for generating lymphoblastoid cell lines (LCLs). This method has been in use for the last two decades with a high success rate. With a somatic mutation rate of 0.3% and ease of cell maintenance, lymphoblas …

淋巴母细胞样细胞系(B lymphoblastoid cell lines,B-L CL),B-LCL在体外能无限增殖( 永生化)[9-11]。B-LCL 是研究EBV致瘤特性的重要细胞模型[12] 。因此,本实验选择了EBV 感染的人B 淋巴细胞,体外细胞

Lymphoblastoid cell lines (LCL) carrying the Epstein-Barr Virus (EBV) are of particular importance. These lines can be established spontaneously from blood and lymphoid tissue from any EBV positive individual by special techniques, and from all individuals by EBV infection of peripheral blood B cells by EBV infection in vitro.

Lymphoblastoid cell lines (LCLs) can be generated easily by in vitro EBV infection of B lymphocytes collected from any individual. In vitro, these EBV-infected B cell cultures yield proliferating, transformed lines referred to as lymphoblastoid cell lines (LCLs).