2021年5月1日 · RCN2 overexpression rescued the inhibitory effect of SP1 inhibitor on HUVEC pyroptosis induced by LPS (all p < 0.05). These findings suggested that the activation of the SP1/RCN2/ROS signaling pathway could promote LPS-induced endothelial cell pyroptosis.

目的:探讨脂多糖诱导HUVEC细胞凋亡的最适浓度和时间.方法:体外培养人脐静脉血管内皮细胞株 (HUVEC),将脂多糖(LPS)分为100,10,1,10-1,10-2,10-3,10-4,10-5 μg·ml-18个浓度,分别刺激0.5,1,2,6,12,24 h后,采用CCK-8法观察细胞活力,荧光显微镜观察Hochest33342/PI双染后细胞形态. …

2017年1月27日 · In this study, LPS-induced inflammatory responses in BEAS-2B human bronchial epithelial cells and human umbilical vein endothelial cell (HUVEC)s were found to be prevented by Dickkopf-1...

摘要: 【目的】研究苜蓿素对脂多糖（LPS）诱导的人脐静脉内皮细胞（HUVECs）炎症损伤的保护作用。 【方法】以HUVECs为研究对象，用MTT法确定苜蓿素的处理浓度，用酶联免疫分析法确定LPS建模浓度以及检测HUVECs炎症相关因子（TNF-α、iNOS、COX-2、IL-1、IL-6）表达，用Western-blot法测定NF-κB和MAPK信号通路蛋白（β-Actin、P65、IKB、P-P65、P-IKB、P38、JNK、ERK1/2、P-P38、P-JNK、P-ERK1/2）表达。

目的研究脂多糖（LPS）对HUVECs的形态和功能的影响，以探讨脂多糖（LPS）活化的血管内皮细胞（VEC）在全身炎症反应，败血症和MOF中的作用。 方法采用体外培养的HUVECs为模型。 倒置显微镜观察LPS对HUVECs形态的影响。 ELISA用于评估IL-6含量。 用共聚焦激光扫描荧光显微镜通过免疫荧光染色法测定ICAM-1的表达。 结果LPS可改变HUVEC的形态，使其呈长绳状。 刺激HUVEC分泌IL-6的LPS的最小剂量为1 ng / ml，与LPS浓度的增加平行地增强了作用，并在 …

2021年2月3日 · We aimed to reveal the effects of macrophages on cell proliferation and apoptosis in LPS induced human umbilical vein endothelial cells (HUVECs). THP-1 derived macrophages and HUVECs were...

2020年6月1日 · 目的 研究大黄素对脂多糖（lps）诱导人脐静脉血管内皮细胞（huvec）氧化损伤的保护作用及机制。方法 运用cck-8细胞活力检测法筛选lps体外诱导huvec细胞氧化损伤模型浓度及大黄素给药浓度。

Endothelial cells play a critical role in inflammation by responding to several endogenous and exogenous proinflammatory stimuli. The three most studied factors that provide inflammatory signals to endothelial cells are lipopolysaccharide (LPS), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β …

LPS treatment promoted wound healing and tube formation in human umbilical vein endothelial cell (HUVEC) cultures and induced their expression of miR-146a. miR-146a was previously shown to regulate angiogenesis in HUVECs.

2023年11月1日 · In this study, the effects of 1 μg/ml of LPS on the metabolism of human umbilical vein endothelial cells (HUVECs) were investigated, and the metabolic changes after LPS stimulation were explained from the perspective of mRNA …