2015年9月16日 · Caspase-11 detects cytosolic bacterial lipopolysaccharide (LPS), playing a critical role in endotoxic shock 7, 8. Caspase-11 and its human counterparts caspase-4/5 are activated by direct...

LPS stimulation 1. Prepare growth medium containing different concentrations of LPS. Use the following conditions: o 0 µg LPS/mL (untreated control) o 0.5 µg LPS/mL o 1 µg LPS/mL o 2 µg LPS/mL 2. Add 250 µL of LPS containing growth medium per well. 3. Incubate cells with LPS for 3 hours at 37°C, 5 % CO 2 and a humidified atmosphere. 4.

2020年6月24日 · Our findings indicate that in human epithelial cells GBP1 acts as a cytosolic LPS sensor and assembles a platform for caspase-4 recruitment and activation at LPS-containing membranes as the...

2015年1月23日 · Here we examine the inflammatory outcome of TNF- and lipopolysaccharide (LPS)-induced necroptosis and report that this mode of cell death resulted in a dramatic suppression of the production of...

2020年3月5日 · Unlike the D275A mutant, GSDMD-AAAD fully restored LPS-induced pyroptosis in GSDMD −/− HeLa cells and showed intact cleavage by endogenous caspase-4 (Figure 3 C). The cleavage product of GSDMD-AAAD resisted detection by the anti-cGSDMD-N antibody due to loss of the antibody epitope.

2014年10月28日 · We demonstrated that LXA 4 could significantly suppress p53, cyclin D1 expression, and migration of LPS-stimulated Hela cells via nuclear factor-κB (NF-κB) pathway, and these effects could be blocked by Boc-2, the specific inhibitor of FPR2/ALX (the receptor of …

We investigated the effect of lipoxin A4 (LXA4) on the proliferation, apoptosis, and migration in lipopolysaccharide (LPS)-stimulated Hela cells. We demonstrated that LXA4 could significantly suppress p53, cyclin D1 expression, and migration of LPS-stimulated Hela cells via nuclear factor-κB (NF-κB) pathway, and these effects could be blocked ...

2016年12月3日 · 我们的结果表明，脂多糖（LPS）与肿瘤细胞上的TLR4结合可以增强HeLa细胞的增殖并增加TLR4和下游分子的表达。用POL-P3b处理HeLa细胞可以降低HeLa细胞的增殖，并以浓度依赖的方式上调Bax水平和下调Bcl-2水平。

方法用lps培养或不培养来自健康供体的表达gr同工型的u-2 os细胞系，不同细胞系（cem，raji，k562和hela）或pbmc。 通过Western印迹评估GRα和GRβ的表达。 在用LPS处理的PBMC中评估糖皮质激素的敏感性，测试被糖皮质激素反式激活或反式抑制的基因。

2020年9月11日 · Inflammatory caspase sensing of cytosolic lipopolysaccharide (LPS) triggers pyroptosis and the concurrent release of damage-associated molecular patterns (DAMPs)....