Inflammation-induced activation of the tryptophan catabolizing enzyme indoleamine 2,3-dioxygenase (IDO) causes depressive-like behavior in mice following acute activation of the innate immune system by lipopolysaccharide (LPS). Here we investigated the mechanism of IDO expression induced by LPS in p …

Here we investigated the mechanism of IDO expression induced by LPS in primary cultures of microglia derived from neonatal C57BL/6J mice. LPS (10 ng/ml) induced IDO transcripts that peaked at 8 h and enzymatic activity at 24 h, resulting in an increase in extracellular kynurenine, the catabolic product of IDO-induced tryptophan catabolism.

2010年8月2日 · Transient stimulation of the innate immune system by an intraperitoneal injection of lipopolysaccharide (LPS) activates peripheral and central expression of the tryptophan degrading enzyme indoleamine 2,3 dioxygenase (IDO) which mediates depressive-like behavior.

采用IDO1活性上调引起的Trp浓度降低、Kyn浓度升高及Kyn/Trp比值增大，来评价IDO1抑制剂L-1-MT和TQ016对急性炎症小鼠血清中上调的IDO1活性的抑制效果。</sec> <sec>结果尾静脉注射0.66 mg/kg LPS或腹腔注射5 mg/kg LPS均显著上调小鼠血清中IDO1活性。

2018年10月29日 · To elucidate the biological role of Ido2 in immune function, we introduced lipopolysaccharide (LPS) endotoxin shock to Ido2 knockout (Ido2 KO) mice, which led to higher mortality than that in the...

We report that peripheral administration of lipopolysaccharide (LPS) activates IDO and culminates in a distinct depressive-like behavioral syndrome, measured by increased duration of immobility in both the forced-swim and tail suspension tests.

2009年11月13日 · In this study, we demonstrated that lipopolysaccharide (LPS) markedly increased nitric oxide (NO) production and indoleamine 2,3-dioxygenase (IDO) activity in mouse peritoneal cells in the presence of activated Vα14 natural killer T cells.

2018年10月29日 · Indoleamine 2,3-dioxygenase 2 (Ido2) is a recently identified catalytic enzyme in the tryptophan-kynurenine pathway that is expressed primarily in monocytes and dendritic cells. To elucidate the biological role of Ido2 in immune function, we introduced lipopolysaccharide (LPS) endotoxin shock to Ido …

2010年2月1日 · LPS (10 ng/ml) induced IDO transcripts that peaked at 8 h and enzymatic activity at 24 h, resulting in an increase in extracellular kynurenine, the catabolic product of IDO-induced tryptophan catabolism. This IDO induction by LPS was accompanied by synthesis and secretion of the proinflammatory cytokines TNFα and IL-6, but without detectable ...

通过动物模型实验显示:在中枢或外周给予脂多糖 (Lippolysaccharide，LPS)、IFN-α等促炎性细胞因子会导致小鼠体内IDO的表达增强，导致抑郁样行为的发生;反之，若提前给予IDO竞争性抑制剂1-MT，可抑制IDO的过度表达，减少抑郁症状的发生;但是，目前因为大多数IDO抑制 ...