2007年8月15日 · Canine monocyte-derived immature DC (iDC) were treated with an exogenous signal, bacterial lipopolysaccharide (LPS), or an endogenous signal, tumor necrosis factor-α (TNF-α), to generate mature DC (mDC).

DC细胞具有巨大的可塑性，既可以发挥免疫激活作用，又可以诱导免疫耐受，具备抗原提呈、免疫耐受、T细胞的极化、调节B细胞以及参与到固有免疫应答反应等多种功能。 由于DC细胞的可塑性，其已成为在诸多临床疾病如移植、肿瘤以及自身免疫疾病等的基本及临床研究的热点。 正常情况下，DC细胞含量较少，人外周血单个核细胞中仅占1%，为满足药物研发需求，赛笠提供未成熟/成熟树突状细胞（iDC/mDC）的诱导服务，助力相关研究的快速推进。

2024年12月10日 · Leptin/LPS-treated DCs increased T cell responses against 4T1 breast cancer in mice. Therefore, in the present study, we investigate the effects of a DC vaccine treated with leptin and LPS on the genes involved in tumor metastasis, angiogenesis, and related cytokines in a mouse model of breast cancer.

2010年1月15日 · We analyzed the kinetics of DC maturation by lipopolysaccharide (LPS) and interferon-γ (IFN-γ) induction in order to characterize the usefulness of mature DCs (mDCs) for immune therapy and to identify biomarkers for assessing the quality of mDCs.

Mononuclear phagocyte genes that were significantly up- or down-regulated in mature DCs (mDCs) produced with 3 different maturation cocktails: LPS and IFN-γ (C1); LPS, IFN-γ and IL-1β (C2); and LPS, IFN-γ, IL-1β and TNF-α (C3)

2018年3月1日 · To obtain mDC, cells were stimulated at day 6 with LPS (serotype EH100, Ra TLR grade, Alexis Biochemicals, 10 ng/ml) for 24 h (hr). Stattic (Sigma-Aldrich) was used at the indicated concentrations (2 and 10 μM) for 25 h, and added to the cells 1 h before LPS.

2017年11月28日 · In this study, we specifically observed the induction of DC maturation by lipopolysaccharide (LPS), which is far upstream from the NF-κB translocation, and also the very start of DC activation mechanism in response to the presence of foreign immunogenic antigens.

Nonylphenol and 4-octylphenol reduced LPS-induced activation of MAPK signaling pathway, MKK1/2 and ERK, concomitant with decreased levels of LPS-induced acetylated histone 4 (H4) at the IP-10 and MDC gene loci.

2023年3月1日 · Breast cancer (BC) was induced in BALB/c mice, and then they received DC vaccine treated with lipopolysaccharide (LPS-mDCs), LPS with a 5 μM dose of CXB (LPS/CXB5-mDCs) and LPS with a 10 μM dose of CXB (LPS/CXB10-mDCs).

In vitro stimulation of monocytes with GM-CSF and IL-4 promotes their differentiation into immature DC (iDC), and further stimulation with LPS induces DC maturation (mDC) [27, 28]. We investigated whether the presence of UC-MSC during the differentiation and maturation phase could alter the general properties of the resulting DC (here indicated ...