The kinetics of the responses of proinflammatory TNFα, anti-inflammatory IL-10, and inflammatory master regulator NF-κB elicited by lipopolysaccharide (LPS) may be critical determinants of the inflammatory response by macrophages; however, there is a …

Our data provide evidence that LPS stimulation of mouse macrophages triggers a selective increase in the levels of gene and protein expression of the immunoproteasomes, resulting in a modulation of specific functional activities of the proteasome and a corresponding increase in NO production as compared to untreated controls.

2019年7月12日 · Here we have demonstrated that in response to LPS and IFN-γ stimulation, metabolic reprogramming in macrophages is highly dynamic. In particular, we revealed a two-stage remodelling of the...

2025年1月22日 · Macrophage-mediated acute inflammation is crucial for pathogen clearance and tissue repair, yet the underlying molecular mechanisms remain inadequately understood. The present study focused on the dynamic profiles of the proteome and phosphoproteome of macrophages exposed to lipopolysaccharide within 1 h.

To understand the synergistic effect of IFN-γ and TNF-α activation, and LPS stimulation on iNOS expression dynamics and NO production, we developed a systems biology based mathematical model. Using our model, we investigated the impact of …

LPS triggers the abundant secretion of many cytokines from macrophages including IL-1 (1), IL-6 (2), and TNF-α (3), which together contributes to the pathophysiology of septic shock. The major cell surface receptor for LPS on macrophages is CD14, a 55-kD glycosyl-phosphatidylinositol–linked membrane protein (4).

2025年2月19日 · Endotoxemia attenuated LPS-induced dynamics more strongly than TNF-α-induced dynamics (Figures 4 C and 4D), while TNF-α was generally more attenuated than LPS in bone-marrow-derived macrophages (Figure 2). These results suggest important differences in how inflammatory stimuli are sensed and converted into NF-κB dynamics in different innate ...

2025年2月19日 · Treatment with TNF-α and LPS revealed clear differences in NF-κB activation dynamics. While nearly all macrophages from saline-treated mice activated following stimulation, response to TNF-α is significantly attenuated, and response to LPS is completely abolished in macrophages from endotoxin-treated mice (Figures 4C–4E).

2018年11月1日 · To address this gap, we used the RAW 264.7 macrophage cell line to define intracellular signaling kinetics and cytokine expression in cells treated with LPS for 15 min to 72 h. The abundance of...

LPS-stimulated NF-κB p65 dynamic response marks the initiation of TNF expression and transition to IL-10 expression in RAW 264.7 macrophages. During injury and infection, inflammation is a response by macrophages to effect healing and repair.