2022年10月1日 · In this study, we developed two LPS morphologies using LPS from Escherichia coli as a model to study the in vitro hydrolytic response when using a lipase treatment. The hydrolysis was performed using lipase b from Candida antarctica to understand the catalytic effect in removing fatty acids from its lipid A moiety on different LPS aggregates.

小角X射线散射 （Small Angle X-ray Scattering, SAXS）是指当X射线照射到试样上，在靠近入射X光束周围2°~5°的小角度范围内发生的散射现象。 由于电磁波的散射遵循 反比定律 ——被照物体的有效尺寸相对于入射波越大，电磁波的散射角越小，因此SAXS的研究主体主要是数百至上千埃的亚微观结构物质（高聚物、蛋白质、溶胶凝胶、超细粉末、多孔碳等）。 总之，SAXS技术凭借其得天独厚的优势，已成为亚微观结构研究的主要工具之一。 图1 X射线与物质的相互作用. …

2023年11月1日 · SAXS combined with wide-angle X-ray scattering (WAXS) experiments revealed significant changes in the layered arrangement of PSUR dispersions upon LPS incubation. The observing broad and unseparated peaks indicated a weaker correlation between sheets and the multilayer structure of PSUR expanded after longer incubation with LPS ( Fig. 9 C).

2025年2月13日 · The outer-membrane lipopolysaccharide (LPS, endotoxin) of gram-negative bacteria is highly immunostimulatory and induces severe pathology in humans. Investigation of natural and reconstituted membrane systems by small angle X-ray scattering (SAXS) revealed that the supramolecular organization and 3D structure of endotoxin molecules are major ...

Transport of lipopolysaccharides (LPS) to the surface of the outer membrane is essential for viability of Gram-negative bacteria. Periplasmic LptC and LptA proteins of the LPS transport system...

2006年4月1日 · The effect of Salmonella minnesota R595 lipopolysaccharide (LPS) on model membrane consisting of a mixture of fully hydrated lipids (dipalmitoylphosphatidylethanolamine (DPPE) and dipalmitoylglycerol (DPG)) was investigated by differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS) and freeze–fracture methods. The DPPE ...

In this work, small-angle X-ray scattering (SAXS) investigation is performed on the ‘melting’ transition of the bicontinuous double diamond cubic phase, formed by pure glycerol monooleate (MO), upon progressive inclusion of varying fractions of pharmaceutical-grade glycerol monooleate (GO) in the hydrated system.

We evaluated the behaviour of a clinically used modified porcine pulmonary surfactant (PSUR) in the presence of LPS in a dynamic system mimicking the respiratory cycle. Polymyxin B (PxB), a...

Small Angle X-ray Scattering (SAXS) is an ideal characterization tool to explore nanoscale systems. In order to investigate nanostructural changes of materials under realistic sample environments, it is essential to equip SAXS with diverse in situ capabilities based on the corresponding requirements.

2017年8月29日 · Transport of lipopolysaccharides (LPS) to the surface of the outer membrane is essential for viability of Gram-negative bacteria. Periplasmic LptC and LptA proteins of the...