Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining has been used extensively to characterize LPS (3, 11, 12). Tsai and Frasch (11) first reported a highly sensitive classic silver staining method for detecting LPS in polyacrylamide gels.

LPS from E coli (Lanes 1A and 1B) and S.typhi (lanes 2A and 2B) was purified by modified hot phenol-water extraction method and fractionated by SDS-PAGE electrophoresis followed by silver (A) or commassie blue staining (B).

2016年7月12日 · In this study, an aptasensor assay based on hybridization chain reaction (HCR) was developed to detect LPS. Briefly, two complementary stable species of biotinylated DNA hairpins coexisted in...

Visualizing LPS banding patterns and recognition by specific antibodies can be useful tools to identify strain lineages and to characterize various mutants. In this report, we describe a hot aqueous-phenol method for the isolation and purification of …

A silver staining method used routinely for detecting bacterial lipopolysaccharide (LPS) in sodium dodecyl sulfate-polyacrylamide gels (C. Tsai and E. Frasch, Anal.

2019年2月27日 · Here we describe an approach based on screening for host LPS alterations caused by selection by bacteriophages. We describe an optimized LPS profiling procedure that is simple, rapid and...

2015年9月15日 · The new imidazole–zinc procedure increases the availability of staining methods while satisfying most of the aforementioned requirements for an ideal stain suitable for the reversible detection of NAGE-separated LPS aggregates and cationic protein–LPS complexes.

LPS was separated on a 5 to 20% gradient polyacrylamide gel in the presence of sodium dodecyl sulfate under reducing conditions, and LPS bands were detected by the silver staining method of...

2001年1月1日 · The lipopolysaccharides are stained dark brown by the silver stain. The silver stain is 500 times more sensitive than the periodic acid-Schiff stain and can detect less than 5 ng of rough type lipopolysaccharides.

2012年7月1日 · As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used silver stain developed by Fomsgaard and coworkers and imidazole–zinc (IZ) negative stain. For its sensitivity and brevity, this stain may be a practical method for LPS determination in the routine laboratory.