Human HEK 293T cells are completely unresponsive to LPS, but acquire high LPS sensitivity following transient transfection with CD14, TLR4, and MD-2 (293T/CD14/TLR4/MD-2 cells), as judged by NF-kappaB activation, ERK 1/2 phosphorylation, and TNF-alpha gene expression.

1999年4月16日 · LPS increases TLR4-mediated NF-κB activation in a dose- and time-dependent manner. HEK 293 cells were transiently transfected with TLR4 cDNA or vector DNA and pELAM-luc.

2022年12月8日 · Strikingly, treatment of glucose/amino acids-deprived HEK-293T cells with E. coli LPS (O111:B4), a complex of lipids and polysaccharides containing β-galactoside residues, led to a significant...

2022年7月1日 · I've been trying to induce an inflammatory response using LPS (Sigma O127:B8 and O55:B5) using the immortalized human myometrial cells (TERT-HM) in our lab but have failed to get a significant...

2023年10月6日 · Upon stimulation with lipopolysaccharide (LPS), the transcription factor NF-κB/p65 enters the nucleus to transactivate genes that express proinflammatory cytokines and NLR family pyrin domain containing 3 (NLRP3), a core …

2015年8月4日 · In this study, we identified a member of the deubiquitinases family, ubiquitin-specific protease 18 (USP18), as a novel negative regulator in Toll-like receptor (TLR)-mediated NF-κB activation in...

We found that overexpression of CD14, TLR4, and MD-2 confers LPS sensitivity to other-wise LPS-unresponsive HEK 293T cells, but does not pre-vent induction of LPS tolerance, despite the lack of down-regulation of TLR4 or MD-2 protein expression by LPS pretreatment.

LPS-induced activation of NF-kB in human embryonic kidney cells (HEK293) (5, 6), whereas in 293T and Ba/F3 cells, TLR4-mediated responses to LPS requires the presence of MD-2, a cell surface protein that associates with TLR4 (7). Observations from studies with TLR2 and TLR4 knockout mice indicate that

2021年10月25日 · In this study LPS from E. coli (Figure 1) was added to HEK-293 cell cultures to evaluate the impact on cell viability and recombinant protein production. The LPS used was a commercial available LPS preparation from the E. coli strain O111:B4, which is frequently used to stimulate immune response [10, 11].

3 天之前 · The LPS-TLR4 immune response is a critical mechanism in the body's defense against Gram-negative bacterial infections, yet its dysregulation can lead to severe inflammatory diseases. Lipopolysaccharide (LPS), a pivotal pathogen-associated molecular pattern (PAMP) on the surface of gram-negative bacteria, is recognized by Toll-like receptor 4 (TLR4), initiating a complex cascade of immune ...