探讨自噬在脂多糖（lipopolysaccharide，LPS）诱导的人肺泡上皮A549细胞炎症反应中的作用及机制。 用LPS刺激A549细胞建立炎症反应模型，按浓度（0、1、5、10 μg/mL）和时间（0、4、8、12、24 ...

目的 探讨自噬在脂多糖（lipopolysaccharide，LPS）诱导的人肺泡上皮A549细胞炎症反应中的作用及机制。 方法 用LPS刺激A549细胞建立炎症反应模型，按浓度（0、1、5、10 μg/mL）和时间（0、4、8、12、24 h）分组（各组<italic>n</italic>=3）。

Pretreatment of A549 cells with NAC significantly ameliorated LPS-caused alterations in caspase-9 activation and DNA damage. Therefore, this study shows that LPS specifically induces apoptotic insults to human alveolar epithelial cells through ROS-mediated activation of the intrinsic mitochondrion-cytochrome c-caspase protease mechanism.

本研究拟采用细菌脂多糖（lps）作为制备ards细胞模型的制模剂，a549细胞模拟Ⅱ型肺泡上皮细胞，探讨在不同浓度、不同作用时间下lps对a549细胞炎性因子〔白细胞介素（il-6、il-1β）、肿瘤坏死因子-α（tnf-α）〕基因表达的影响，以期为进一步确定制备ards细胞 ...

2010年9月17日 · Pretreatment of A549 cells with NAC significantly ameliorated LPS-caused alterations in caspase-9 activation and DNA damage. Therefore, this study shows that LPS specifically induces apoptotic insults to human alveolar epithelial cells through ROS-mediated activation of the intrinsic mitochondrion–cytochrome c-caspase protease mechanism.

2020年3月1日 · In the present study, we used LPS to induce the inflammatory injury of A549 cells, and investigated the role of miR-297 in LPS-induced A549 cells. We demonstrated LPS effectively suppressed the expression of miR-297 and cell viability, and promoted apoptosis of A549 cells in vitro, as well as increased the expressions of inflammatory cytokines ...

As LPS could upregulate cGAS expression in A549 cells, the authors hypothesized that LPS might enhance DNA-induced IFN-β production. To test this hypothesis, A549 cells were pretreated with LPS for 4 h. Then, LPS was removed, and cells were transfected with DNA. The total IFN-β levels were measured using ELISA 24 h post-transfection.

目的 研究肺泡Ⅱ型上皮细胞 A549 对 TNF-α 和脂多糖(lipopolysaccharides, LPS)刺激的反应效果,探讨急性肺损伤体外细胞模型最佳造模方法. 方法 体外培养的 A549 细胞分为正常对照组 (normal control, NC 组),10 ng/ml TNF-α 刺激 2 h 组,10 ng/ml 和 100 ng/ml LPS 分别刺激 2,4,8,16 h 组.

YKL-40 knockdown in LPS-treated A549 cells significantly decreased the production of pro-inflammatory cytokines and reduced cell apoptosis, whereas it concomitantly caused upregulation of Bax and downregulation of Bcl-2, cleaved caspase-3 and cleaved caspase-9.

目的研究自噬在脂多糖（lps）诱导人肺泡上皮a549细胞炎症反应中的作用及机制。方法 用lps刺激a549细胞建立炎症反应细胞模型，然后按浓度（0、1、5、10 μg/ml）和时间（0、4、8、 12 小时和 24 小时）。