CD14 是一种糖磷脂酰肌醇锚定的质膜蛋白，在 LPS 介导的 TLR4 激活中具有长期确定的作用；CD14 与 LPS 结合并将 LPS 转移到 TLR4-MD2 复合物，从而启动 myddosome 组装和信号转导（Lipopolysaccharide induces alveolar macrophage necrosis via CD14 and the P2X7 receptor leading to interleukin-1α release.

CD14，即 LPS （Lipopolysaccharide）受体，最初是一种存在于 单核细胞 、 巨噬细胞 等 细胞表面 的 白细胞 分化抗原，由TODD于1981年首次从人单核细胞表面发现。

cd14是革兰阴性菌内毒素脂多糖lps和lps结合蛋白复合物的高亲和力受体。cd14识别并结合lps，引起细胞酪氨酸磷酸化、核因子nf-κb转位、触发细胞因子释放和氧自由基产生，在机体免疫、防御系统引起的一系列反应中发挥重要作用。

2019年8月1日 · CD14 binds to Peptidoglycan (PGN) with partially identical conformational binding sites on CD14 compared with LPS and then leads to TLR2-mediated transcription factors NF-κB, CREB/ATF and AP-1 activation in human and mouse monocytes to …

2018年6月29日 · The secretory form of the CD14 (sCD14) induced internalization but required a much higher concentration than LBP. An inhibitory anti-sCD14 mAb was ineffective for serum-mediated internalization. LBP lacking the domain for LPS transfer to CD14 and a CD14 mutant with reduced LPS binding both attenuated TLR4 internalization.

LPS stimulation of CD14-expressing cells induced TLR4 internalization in the presence of serum, and an inhibitory anti-LBP mAb blocked its internalization. Addition of LBP to serum-free cultures restored LPS-induced TLR4 internalization to comparable levels of serum.

Collectively, these data establish that the natural coexpression of CD14 and TLR4 determines which cell types internalize TLR4 in response to LPS. CD14 mediates the LPS-induced internalization of extracellular macromolecules. In addition to promoting TLR4 endocytosis, LPS induces DCs to undergo rapid macropinocytosis (West et al., 2004). To ...

2023年12月1日 · CD14, a co-receptor for TLR4, recognizes LPS, PAMPs, and DAMPs in innate immunity. CD14 has a role in TLR4-dependent and TLR4-independent signaling pathways. CD14 helps clear apoptotic cells by recognizing phospholipids externalized on the cell surface. In barrier tissues, CD14 can mediate inflammatory and tolerogenic responses.

2022年5月3日 · Our data show that LPS internalization via CD14 is a consequential event that enables cytosolic translocation of LPS, licensing pyroptotic and IL-1 responses from the host.

1990年9月21日 · CD14, a differentiation antigen of monocytes, was found to bind complexes of LPS and LBP, and blockade of CD14 with monoclonal antibodies prevented synthesis of TNF-alpha by whole blood incubated with LPS. Thus, LPS may induce responses by interacting with a soluble binding protein in serum that then binds the cell surface protein CD14.