LAMP1 was fused to a SBP and a fluorescent protein in its luminal domain and transiently expressed in HeLa cells. In the absence of biotin, SBP-mCherry-LAMP1 was retained in the ER, thanks to the coexpression of the ER-resident Streptavidin-KDEL (Supplemental Figure S1a).

2023年4月6日 · HeLa cells expressing doxycycline-inducible LAMP1–APEX2 were treated with LLOMe followed by exposure to biotin and H 2 O 2.

2020年1月6日 · Here, we show that galectin-3 (Gal3), a β-galactoside-binding cytosolic lectin, unifies and coordinates ESCRT and autophagy responses to lysosomal damage. Gal3 and its capacity to recognize damage-exposed glycans were required for efficient recruitment of the ESCRT component ALIX during lysosomal damage.

研究人员首先通过荧光探针监测u2os细胞，发现其溶酶体蛋白水解活性在饥饿反应中显著增加。lamp1标记的溶酶体在hela和u2os细胞中在核周围聚集，并且大部分不运动。在饥饿细胞的核周区域电子密集度大的可能是降解的溶酶体。

Using a series of specific protocols we show that LAMP1–YCaM expressed in HeLa cells responds to Ca 2+ signals generated by extracellular application of the physiological agonist histamine. Furthermore we demonstrate that histamine responses persist in cells pre-treated with the ER SERCA (sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase) pump ...

Furthermore, we immunostained endogenous LAMP1 in HeLa, HepG2, and HCT116 cells and performed flow cytometry. We found that LAMP1 staining was significantly increased in cells at the S and G2/M phases compared with cells at the G1 phase (Fig. 6, C and D). Collectively, these findings indicated that lysosome biogenesis is increased in the S and ...

2020年1月6日 · Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines.

2025年2月27日 · HeLa cells were exposed to U18666A (2.5 μM) in the absence or the presence of SFN (15 μM) for 24 h. Each panel shows fluorescence images taken by confocal microscopes. The red signal is LAMP1-mCherry driven by stable transfection, and the green signal is Filipin.

摘要: 目的：探究罗哌卡因对HeLa细胞自噬及自噬溶酶体的影响方法：采用HeLa、HeLa-GFP-LC3、HeLa-RFP-LAMP1等细胞系进行研究，并将细胞分为空白对照组、饥饿组（饥饿处理0、4、12 h）和罗哌卡因组（10 mg/mL罗哌卡因，处理0、4、12 h），使用显微成像技术联合多种生化 ...

Others have used a Ca 2+ sensor targeted to lysosomal membranes (LAMP1-yellow cam-eleon3.6) to detect Ca 2+ signals evoked by histamine in HeLa cells (McCue et al., 2013), but this sensor with...