2018年9月21日 · Dynamic binding capacity (DBC) is key for choosing the resin that will be the most suitable for purifying your protein sample. DBC is available in the technical specifications for Cytiva columns and resins, for typical proteins and running conditions.

纤维层析基质（例如 Fibro PrismA）结合大量蛋白的时间只有亲和填料所需纯化时间的几分之一。与填料一样，Fibro 单元的动态结合载量 (DBC) 会受到工艺条件和目标分子性质的影响。因此，测定 Fibro 单元的动态结合载量是一个非常关键的工艺步骤。

2023年4月2日 · Dynamic binding capacity (DBC) indicates the quantity of a specific biomolecule that can be bound during a chromatography purification step under a specific set of process conditions. Determination and optimization of this value facilitates the development of efficient and productive chromatography operations.

2021年6月29日 · The SST 8300 utilizes a unique combination of vacuum and gas pressure above atmospheric, to reduce voids close to 1%. This method is ideally suited for large surface areas, such as DBC to base plate soldering.

2022年2月2日 · DBC is defined as the amount of a target protein bound to the resin under determined flow conditions before significant breakthrough of unbound protein occurs. 14 DBC is determined by loading a sample of the desired protein at target flow conditions and monitoring the flow through until the target protein reaches break point, and unbound ...

2018年11月2日 · The DBC of a chromatography resin is the amount of target protein that binds to the resin under given flow conditions before a significant breakthrough of unbound protein occurs. DBC is determined by loading a sample containing a known concentration of the target protein and monitoring the flow-through.

High productivity is the main focus when a chromatography resin is selected in downstream processing (DSP). The higher the dynamic binding capacity (DBC) of a resin, the more substances can be loaded onto the column and consequently the higher is …

Fiber chromatography matrices such as Fibro PrismA can bind a large amount of protein in a fraction of the purification time required for an affinity resin. Just like with resins, the dynamic binding capacity (DBC) of a Fibro unit is affected by process conditions and by the properties of the target molecule.

The IEX resins from YMC BioPro IEX exhibit high DBC values for various types of biomolecules – from small peptides to large proteins and antibodies – even at elevated flow rates. In a direct comparison of the specified DBC values, the resins from YMC clearly outperform alternative resins used for process scale. Conclusion

2022年9月3日 · Dynamic Binding Capacity (DBC), also known as Protein Binding Capacity (PBC) or target volume, measures the amount of target protein that a particular affinity resin can capture.