Biophysical basis underlying dynamic Lck activation visualized by ...
Our results show that Lck-FRET-Zap70FY and Lck-FRET-ITAM1 exhibited the largest increase in ECFP/FRET emission ratio toward Lck kinase activity, while Lck-FRET-Zap70YY was …
We developed a new and sensitive FRET biosensor (ZapLck) to visualize Lck kinase activity with high spatiotemporal resolutions in live cells. ZapLck revealed that 62% of Lck signal was …
T Cell Activation Results in Conformational Changes in the Src …
2013年2月19日 · We applied a Levenberg-Marquardt nonlinear, least-squares algorithm to dissect the decay kinetics of ECFP fluorescence in the Lck biosensor into individual lifetimes. A three …
Lck-FRB-ECFP(W66A) - Addgene
Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)
Plasma membrane restricted RhoGEF activity is sufficient for …
2015年10月5日 · RFP-cDH or RFP constructs were fused to an FKBP12 domain, which, upon addition of rapamycin, will be recruited to Lck-FRB-ECFP (W66A). Lck-FRB-ECFP (W66A) is …
Low level of Lck kinase in Th2 cells limits expression of CD4 co ...
2017年5月24日 · Ectopic overexpression of Lck in Th2 cells results in increased expression of CD4 co-receptor and enhanced S73 phosphorylation of transcription factor c-Jun. Our findings …
Reversible chemical dimerizer-induced recovery of PIP2 levels …
2015年6月15日 · At around 80% confluence, HeLa Kyoto cells were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. 1 μg DNA was …
Lck kinase activities regulated by Y394 in T cells ... - ResearchGate
We developed a new and sensitive FRET biosensor (ZapLck) to visualize Lck kinase activity with high spatiotemporal resolutions in live cells. ZapLck revealed that 62% of Lck signal was...
Biophysical basis underlying dynamic Lck activation visualized …
2019年6月1日 · Lck plays crucial roles in TCR signaling. We developed a new and sensitive FRET biosensor (ZapLck) to visualize Lck kinase activity with high spatiotemporal resolutions …
Recruitment of a splicing factor to the nuclear lamina for its ...
2022年7月22日 · Whereas rCD1-dependent recruitment to the PM or the endomembrane system (anchor proteins Lck-ECFP-SNAP or ECFP-SNAP-CAAX, respectively) was not successful, …