The Km values for heteroterameric H2M2-mediated catalysis of pyruvate or lactate are between those for the homotetrameric isozymes, M4 and H4, whereas the Vmax values are similar. The Km and Vmax values for H2M2-mediated catalysis …

We determined the Michaelis constant (K (m)) and maximal velocity (Vmax) of lactate dehydrogenase (LDH) in periportal hepatocytes and skeletal muscle fibers by three different histochemical assay methods.

2019年3月18日 · LDHA needs a higher pyruvate concentration for the maximum enzyme activity. It means that the Michaelis constant (Km) of LDHA for pyruvate is 3–10 fold greater than the Km calculated for...

2023年3月24日 · Isoenzyme divergence is a prevalent mechanism governing tissue-specific and developmental stage-specific metabolism in mammals. The lactate dehydrogenase (LDH) isoenzyme spectrum reflects the tissue-specific metabolic status. We found that three tetrameric isoenzymes composed of LDHA and LDHB (LDH-3/4/5) comprise the LDH spectrum in T cells.

2024年1月5日 · The ROC and Kaplan-Meier (KM) curves, independent prognostic factors, and clinical correlation analyses were employed to identify the prognostic potential of LDHA using the R platform. Gene set enrichment analysis (GSEA) was performed to elucidate the key functions regulated by LDHA.

2018年1月1日 · This isoform has the highest activity catalyzing the reaction toward formation of lactate and NAD +, and it was reported that at pH 6.0, it displays a KM for pyruvate around three to tenfold higher than the H4 isoform (Read et al. 2001). Hence, LDHA presents a key role on energetic metabolism.

2024年8月10日 · Lactate dehydrogenase A (LDHA) is highly expressed in many tumor cells and promotes the conversion of pyruvate to lactic acid in the glucose pathway, providing energy and synthetic precursors for rapid proliferation of tumor cells. Therefore, inhibition of LDHA has become a widely concerned tumor treatment strategy.

2024年3月6日 · ldh是葡萄糖代谢中的关键参与者，调节丙酮酸和乳酸之间的转化。ldh由两个主要亚基组成（ldha和ldhb），其中ldha在缺氧条件下将丙酮酸转化为乳酸，而ldhb在氧气存在时有利于乳酸转化为丙酮酸。

2024年12月25日 · LDHA (Lactate Dehydrogenase A) is a Protein Coding gene. Diseases associated with LDHA include Fanconi-Bickel Syndrome and Tango2-Related Metabolic Encephalopathy And Arrythmias. Among its related pathways are glycolysis (BioCyc) and Regulation of pyruvate metabolism.

2021年7月5日 · Here we show that oxaloacetate (OAA) is a competitive inhibitor of human lactate dehydrogenase A (LDHA) and that elevated PKM2 activity increases de novo synthesis of OAA through glutaminolysis,...