The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

As a key cell surface receptor for cholesterol uptake and traffic, low-density lipoprotein receptor (LDLR) is susceptible to be attacked by detrimental factors. The LDLR regulates the homeostasis of plasma and intracellular cholesterol through intaking plasma-derived LDL cholesterol and mediating its internalization and metabolism.

2019年8月6日 · The 120 kDa CTF was detected in the livers from humans and mice expressing human LDLR. Collectively, these results identify that BMP1 regulates cellular LDL uptake and may provide a target to...

2024年7月23日 · Like other secretory and endomembrane proteins, nascent LDLR is initially synthesized in the ER as an immature protein with a molecular weight of ∼120 KDa and is post-translationally modified by N- and O-glycosylation in the ER and the Golgi apparatus increasing its apparent molecular weight from ∼120 KDa to ∼160 KDa (Omer, 2018).

The main well-known LDLR ligand is represented by apolipoprotein E (ApoE), a 34 kDa soluble protein that is a main component of lipoproteins in plasma and a secreted glycoprotein of 34 kDa that acts as a cholesterol carrier and signaling molecule.

2023年7月14日 · The low-density lipoprotein (LDL) receptor (LDLR) is a membrane glycoprotein with a molecular mass of 160 kDa, that mediates the uptake of lipoprotein particles, mainly LDL, into cells via receptor-mediated endocytosis [5].

2024年9月15日 · Hepatic LDLR abundance is a central determinant of lipoprotein clearance. Dysregulation of the LDLR pathway is associated with hypercholesterolemia and development of atherosclerosis. Emerging post-transcriptional mechanisms that govern LDLR abundance offer potential new lipid-lowering strategies.

LDLR 蛋白与 LDL 关键结合，通过内吞作用转运胆固醇。 聚集成网格蛋白包被的小凹坑启动内化。 与 DAB2、LDLRAP1、ARRB1、SNX17 和不成熟的 PCSK9 的相互作用有助于其功能的多功能性。 酪氨酸磷酸化会损害 NPXY 基序相互作用。

Proprotein convertase subtilisin/kexin type 9 (PCSK9) binds to the LDL receptor (LDLR) at the cell surface and reroutes the internalized LDLR to intracellular degradation. In this study, we have shown that PCSK9-mediated degradation of the full-length 160 kDa LDLR generates a 17 kDa C-terminal LDLR …

The low-density lipoprotein receptor (LDLR) is responsible for transporting cholesterol-containing lipoprotein particles from the circulation into cells. Patients with loss-of-function mutations in the LDLR gene have familial hypercholesterolemia (FH), an autosomal dominant genetic disorder affecting ∼1 in 500 individuals worldwide.

2017年2月22日 · To study the impact of mutations affecting the hydrophobic transmembrane domain of the LDLR, each of the 22 amino acids of the transmembrane domain was individually mutated to arginine. The more centrally in the transmembrane domain an arginine was located, the lower amounts of the 120 kDa precursor LDLR in the endoplasmic reticulum were observed.

2022年7月1日 · Relative band intensities of mature (≈160 kDa) and precursor (≈120 kDa) forms of LDLR protein were quantified by densitometric analysis using Image Lab Software (BioRad) and normalized to levels of α-tubulin.

2012年11月25日 · Proprotein convertase subtilisin/kexin type 9 (PCSK9) binds to the LDL receptor (LDLR) at the cell surface and reroutes the internalized LDLR to intracellular degradation. In this study, we have shown that PCSK9-mediated degradation of the full-length 160 kDa LDLR generates a 17 kDa C-terminal LDLR fragment.

3 天之前 · This type of oxLDL can induce the formation of foam cells and therefore is widely used to study lipid metabolism and atherosclerosis. Recombinant human CD36, LDL receptor (LDLR), and anti-CD36 and anti-LDLR antibodies were from Abcam (Cambridge, MA).

1 天前 · Introduction Cardiovascular disease is a major global health issue, and atherosclerosis is a leading cause of cardiovascular conditions. Traditional approaches for managing atherosclerosis have limitations, creating a need for alternative preventive strategies such as vaccines. Methods The authors conducted a systematic review following Cochrane Handbook and PRISMA guidelines. They searched ...