2022年7月1日 · Here, using RNA in situ hybridization and/or immunohistochemistry, we thoroughly investigated LGR5 expression in normal human ovaries, fallopian tubes and various ovarian tumors. We...

Using in vivo lineage tracing, we identify embryonic and neonate Lgr5(+) populations as stem/progenitor cells contributing to the development of the OSE cell lineage, as well as epithelia of the mesovarian ligament and oviduct/fimbria. Adult Lgr5(+) populations maintain OSE homeostasis and ovulatory regenerative repair in vivo. Thus, Lgr5 marks ...

RT-PCR results showed that Lgr5 was expressed in the ovary (Fig. 6A), and deletion of Lgr5 by Pgr-Cre was confirmed by PCR analysis of genomic DNA (Fig. 6B) and mRNA (Fig. 6A). Using in situ hybridization, we observed that Lgr5 was primarily localized in the CL and ovarian surface epithelium in Lgr5 f/f females.

2018年5月18日 · Leucine‐rich repeat‐containing G protein‐coupled receptor 5 (LGR 5) plays a vital role in the development of malignant tumors; however, its biological role and underlying mechanism in epithelial ovarian cancer (EOC) remain unclear. In this study, we aimed to investigate the biological function and clinical significance of LGR 5 in human EOC.

2014年8月1日 · Lgr5, a marker of stem cells in many organs, is found expressed in cells scattered through the ovarian epithelium that expand to repair and regenerate the damaged ovarian surface after each...

2014年7月6日 · Lgr5 marks stem cells in multiple epithelia. Here we use reporter mice and single-molecule fluorescent in situ hybridization to document candidate Lgr5 + stem cells in the mouse ovary and...

Here, using RNA in situ hybridization and/or immunohistochemistry, we thoroughly investigated LGR5 expression in normal human ovaries, fallopian tubes and various ovarian tumors. We discovered that LGR5 expression is negligible in the human ovary surface epithelium, whereas ovarian stromal cells normally express low levels of LGR5.

2018年1月2日 · LGR5 and LGR6 have gained prominence because they are among the few known stem cell-specific receptors. Studies in mice have shown that LGR5 marks epithelial stem cells in the intestine, hair follicle, kidney, mammary gland, colon, and ovary [9–13]; LGR6 marks stem cells in the epidermis, mammary gland, nail, and fallopian tube [14–17]. The ...

Of interest, P4 supplementation rescued decidualization failure and supported pregnancy to full term in Lgr5(d/d) females. These results provide strong evidence that Lgr5 is critical to normal CL function, unveiling a new role of LGR5 in the ovary.

2022年7月1日 · Here, using RNA in situ hybridization and/or immunohistochemistry, we thoroughly investigated LGR5 expression in normal human ovaries, fallopian tubes and various ovarian tumors. We discovered that LGR5 expression is negligible in the human ovary surface epithelium, whereas ovarian stromal cells normally express low levels of LGR5.