
Single-particle cryo-EM structures from iDPC–STEM at near …
2022年9月5日 · Integrated differential phase contrast (iDPC)–STEM imaging of keyhole limpet hemocyanin and tobacco mosaic virus enabled cryo-EM structure determination at 6.5 and 3.5 Å resolution, respectively.
Imaging biological samples by integrated differential phase …
2022年3月1日 · Recently, integrated differential phase contrast (iDPC) STEM technique emerged and achieved a linear phase contrast imaging condition, while resolving signals of light elements next to heavy ones even at low electron dose. This enables successful investigation of beam sensitive materials.
时下火热的iDPC技术,究竟为何? - 知乎 - 知乎专栏
研究利用iDPC技术,直接在原子尺度上观察到了Bi2Sr2CaCu2O8+δ中掺杂氧原子的位置(如下图所示)。 结果表明,室温下掺杂氧原子位于BiO和SrO原子层中间的张应力区域,并呈现不均匀分布,有的区域出现成对氧原子,有的区域呈现单个氧原子。
Imaging biological samples by integrated differential phase …
Recently, integrated differential phase contrast (iDPC) STEM technique emerged and achieved a linear phase contrast imaging condition, while resolving signals of light elements next to heavy ones even at low electron dose. This enables successful investigation of …
Differential phase contrast STEM image calculation software – …
2024年12月1日 · Magnifier is the first publicly available software for modeling DPC-STEM images. The capability of Magnifier to reproduce experimental DPC-STEM images was confirmed by comparing experimental iDPC-STEM images of Li5OsO6, α …
清华大学材料科学与工程研究院《材料科学论坛》学术报 …
A state of the art direct phase STEM imaging technique called Integrated Differential Phase Contrast STEM (iDPC-STEM) [1, 2] will be presented, both from fundamental and theoretical point of view...
Phase Transitions and Ion Transport in Lithium ... - Wiley Online …
2024年6月10日 · Our findings ultimately clarify the mechanism of Li storage in LFP at the atomic level and offer direct visualization of lithium dynamics in this material. Supported by multislice calculations and EELS analysis we thereby offer the most detailed insight into lithium iron phosphate phase transitions which was hitherto reported.
The integrated differential phase contrast (IDPC) method is useful for generating the potential map of a thin sample. We evaluate theo-retically the potential of IDPC imaging for thick samples by varying the focus at different sample thicknesses. Our calculations show
化工系魏飞团队利用低剂量相位衬度电镜技术助力MOF原子级成像 …
针对这一问题,清华大学化工系魏飞教授课题组借助积分差分相位衬度技术(iDPC-STEM),解决低电子束流下图像分辨率和信噪比低的问题,并通过控制电子剂量,以非常高的分辨率和信噪比对MIL-101,一种稳定的MOF材料,实现了原子级成像,分辨率可达1.8埃(图1所示),是目前文献报道中此材料的最高分辨率。...
[PDF] Imaging biological samples by integrated differential phase ...
2022年1月1日 · Optimizing experimental parameters of integrated differential phase contrast (iDPC) for atomic resolution imaging. Data show that STEM imaging in general, and in particular the iDPC–STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.