2018年10月30日 · We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these...

2023年11月1日 · We combined Acr with the light-oxygen-voltage 2 (LOV2) photosensor from Avena sativa according to previous studies (Supplementary Figure S4A) (29, 30). We also optimized the PICI system to simplify the previous procedures by redesigning the complete plasmid for expressing Cas9 and Acr using araBAD and …

We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.

2023年7月1日 · More recently, a separate group showed that circularly permuted LOV2 (cpLOV2, which can provide distinct “caging surfaces” relative to the non-permuted domain) can also control anti-CRISPR functions, 39 affording additional options for effective fusions to the ever-growing roster of Acr proteins.

通过 诱导型启动子 、光或小分子调控Acr蛋白，可以实现CRISPR-Cas活性的快速、动态和空间控制。例如，将AcrA4II融合到光敏LOV2结构域，使得抗CRISPR活性可以被蓝光控制(Bubeck等,2018)。 降低CRISPR-Cas编辑在组织中的细胞毒性

2021年5月6日 · We demonstrate the use of cpLOV2-based optogenetic tools to reversibly gate ion channels, antagonize CRISPR–Cas9-mediated genome engineering, control protein subcellular localization, reprogram...

We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR–Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.

We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inh... ... Acr proteins like AcrIIA4 and AcrIIA5 inhibiting SpCas9, and all Cas9...

Acr proteins can be modified for specific laboratory applications, e.g., a hybrid of AcrIIA4 with a light-induced LOV2 domain has been shown to control genome and epigenome editing mediated by...

黑暗条件下AsLOV2 (Arabidopsis sativa LOV2)或AtLOV2 (Arabidopsis thaliana LOV2)结构域与C末端螺旋Jα结合；蓝光激活条件下，由于半胱氨酸残基的高度保守，使LOV核心与黄素蛋白FMN之间的非共价作用转变为共价作用，由此发生光诱导构象变化，解除Jα螺旋结构对LOV核心结 …