Learn how to phenotype LSK, LSK/SLAM, and ESLAM hematopoietic stem and progenitor cells from mouse bone marrow using flow cytometry.

cell population is also termed LSK. Less than 10% of LSK cells are long-term HSCs (LT-HSCs).3 Two main strategies are commonly used to identify mouse LT- HSCs within the LSK cell population: 1) LSK cells with no or only weak expression of CD34 and CD135⁴,⁵ 2) LSK cells with expression of CD150 and no expression of CD48⁶

摘要:造血干细胞 (Hematopoietic Stem Cells, HSCs) 是具有长期自我更新,以及分化为各类成熟血细胞能力的成体干细胞。 本文以小鼠骨髓组织为例,使用7 色共14种抗体,标记出在骨髓细胞中的造血干细胞。 用此法标记的HSCs还可进行细胞周期、凋亡等实验,并可用于分选实验。 表1. 流式抗体. 7. 同型对照及单标抗体, 见表2. 表2. 同型及单标抗体. 8. 磷酸缓冲液 (10x PBS, Sangon biotech, catalog number: E607016-0500) 9. 氯化铵 ( 天津大茂化学,catalog number: 12125-02-9) 10.

The hematopoietic progenitor population was initially refined using a light scatter gate followed by discrimination of doublets and dead cells. Lin+ cells were excluded, followed by gating on the Sca1+c-Kit+(LSK) population. CD150+CD48- cells within the LSK population were identified and quantitated in the final gating step.

2012年6月26日 · Regarding where to draw the SP gate, particularly how far towards the top of the SP, we prefer a conservative SP gate, while attempting to maximize cell number yield and minimize contamination from non-HSCs. Mouse SP cells typically comprise about 0.01% of whole bone marrow within a gate excluding dead cells and red blood cells.

2018年7月24日 · Importantly, 24 h post stimulation, the increase in Sca1 shifted almost the entire Lineage - cKit + Sca1 − (LK) population into the Lineage − cKit + Sca1 + (LSK) gate (Fig. 1a, b, Supplementary...

2008年11月20日 · Multipotent long-term HSCs (LT-HSCs) reside in the bone marrow and through a process of asymmetric cell division, can self-renew to sustain the stem cell pool or differentiate into short-term HSCs (ST-HSCs) or lineage-restricted progenitors that undergo extensive proliferation and differentiation to produce terminally differentiated, functional ...

... a plot of PECy7, set CD150 + gate under Sca-1 + c-Kit + gate (Gate 2) to include clearly separated, the most positive population. Normally 20%-30% of CD48 -LSK cells are expected to be...

LSK细胞就是小鼠骨髓中的造血干细胞。 从小鼠后退骨中提取出细胞，然后染色，上流式细胞仪，最后Gate出lsk细胞就认为是造血干细胞。

2022年10月19日 · One of the biggest challenges in HSC analysis postirradiation is HSC gating by flow cytometry. Widely established gating strategies for HSCs from mouse bone marrow begin with an LSK gate (Lineage-(see Note 1), Sca-1 +, c-Kit +), which contains HSCs as well as their earliest progeny [1,2,3,4].