2017年8月10日 · Mice bearing Luc-expressing tumors developed IFN-γ response to the dominant CTL epitope of Luc. Induced by intradermal DNA-immunization, such response protected mice from the establishment...

2020年2月7日 · 刚好看到这篇文献，PER2::LUC 是将荧光素蛋白定点knockin到小鼠自身Per2基因3’端，形成PER2-LUC的融合蛋白，以荧光强度来报告昼夜周期。 通常，更常用的荧光报告转基因小鼠策略是使用目的基因UTR区的启动子（promoter）和增强子连接荧光素序列。 当目的基因表达时启动子、增强子会顺便激活荧光素基因的表达，就可以报告目的基因的转录强弱。 但是Per1基因使用该策略不能报告外周组织的转录水平的昼夜节律，所以这篇文献的研究者采用了敲入荧光 …

2024年6月13日 · To monitor tumor growth, cancer cells that are implanted in mice are often transfected with reporter genes, such as firefly luciferase (Luc), enabling in vivo optical imaging over time.

006852 B6.129S6- Per2 tm1Jt /J These targeted reporter mutant mice express PER2::LUC bioluminescence similar to endogenous Per2 expression, and may be useful as a real-time reporter of circadian dynamics in different tissues.

2023年9月7日 · Here we created two Cre-dependent reporter mice incorporating luciferases oFluc derived from Pyrocoeli matsumurai and Akaluc, both of which had been reported previously to be brighter than Luc2...

2008年12月1日 · Using this mouse model, we describe a facile assay that enables the assessment of the pharmacodynamics of a systemically delivered siRNA formulation. These mice can also be used as universal donors, enabling the efficient and sensitive monitoring of cell trafficking or tissue transplantation.

2013年1月17日 · Here, we describe a luciferase knockin mouse (p16(LUC)), which faithfully reports expression of p16(INK4a), a tumor suppressor and aging biomarker. Lifelong assessment of luminescence in p16(+/LUC) mice revealed an exponential increase with aging, which was highly variable in a cohort of contemporaneously housed, syngeneic mice.

利用Chicken-ß-actin系统性 强启动子 建立的全身表达荧光素酶（Luciferase）的 转基因小鼠 可以利用光学成像技术跟踪靶细胞，是研究干细胞、肿瘤细胞的工具小鼠。 1月龄luc转基因小鼠腹腔注射底物 D-Luciferin 溶液（25mg/ml），达到150µg/kg，10min 后，用饱和 三溴乙醇 麻醉，放入活体荧光影像系统的暗箱中采像，SlideBook 4.0 软件进行分析，luc转基因小鼠发出荧光。 荧光素酶是生物体内催化荧光素 (Luciferin)或脂肪醛 (Firefly Aldehyde)氧化发光的一类酶的总称。 它来 …

2019年4月24日 · To facilitate the identification of molecular pathways of Socs3 induction, we generated a real-time gene expression reporter mouse of Socs3 (Socs3-Luc mice). We successfully detected a remarkable increase in luciferase activity in various tissues of Socs3 -Luc mice in response to a peripheral injection of lipopolysaccharide, a potent inducer of ...

Monitoring cancer and aging in vivo remains experimentally challenging. Here, we describe a luciferase knockin mouse (p16LUC), which faithfully reports expression of p16INK4a, a tumor suppressor and aging biomarker.