2024年6月27日 · To gain insight into endothelial cell alterations in lung fibrosis we performed single cell RNA-sequencing of bleomycin-injured lungs from young and aged mice. Analysis reveals activated cell...

In this study, we optimized magnetic- and fluorescence-based isolation protocols to segregate lung epithelial (CD326/epithelial cell adhesion molecule–positive), endothelial (CD31-positive), and immune (CD45-positive) cells, with high purity, from the lungs of transgenic mice with mutant epidermal growth factor receptor–induced lung ...

2021年4月16日 · To ensure isolated CD31+/podoplanin + cells were LECs, we examined expression of the lymphatic-specific cell markers podoplanin, prospero homeobox protein 1 (PROX-1) and lymphatic vessel...

CD31 signals confer immune privilege to the vascular endothelium. Proc Natl Acad Sci U S A. 2015;112:E5815–5824. doi: 10.1073/pnas.1509627112. This study builds importantly on the results of reference 65 to demonstrate that PECAM-1 ITIM signaling confers cytoprotection to the vascular endothelium exposed to pro-apoptotic stimuli.

The present study aimed to characterize CD45 − /CD31 + LSP cells in the adult mouse lung, to clarify their differentiation potential, and to examine the association between these two types of LSP cells in vitro. Materials and methods Animals

Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2‑ and VEGFR2+ cells. The CD45‑/CD31+/VEGFR2‑ LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro.

CD31 responds more sensitive to anlotinib treatment than CD105 in HUVEC cells via PI3K‐AKT pathway. A, CD31 protein was down‐regulated by Western blot analysis following the anlotinib treatment at various concentrations.

2021年9月17日 · Timing: ∼5 h For preparation of lung digestion buffer, 30 min. For digestion of lung tissue, 1 h. For immune and epithelial cell depletion using CD45 and EpCAM murine MicroBeads, 45 min. For endothelial cell enrichment using …

从成年小鼠肺中分离得到的sp细胞可分为cd45 +亚群（骨髓来源）和cd45-亚群，可细分为cd31-和cd31 +亚群。cd45‑ / cd31‑肺sp（lsp）细胞是间充质干细胞。但是，cd45‑ / cd31 + lsp单元尚未完全表征。在本研究中，发现cd45‑ / cd31 + lsp细胞能够形成菌落。

In this study, we optimized magnetic- and fluorescence-based isolation protocols to segregate lung epithelial (CD326/epithelial cell adhesion molecule-positive), endothelial (CD31-positive), and immune (CD45-positive) cells, with high purity, from the lungs of transgenic mice with mutant epidermal growth factor receptor-induced lung ...