Escherichia coli transports lysine by two distinct systems, one of which is specific for lysine (LysP) and the other of which is inhibited by arginine ornithine. The activity of the lysine-specific system increases with growth in acidic medium, anaerobiosis, and high concentrations of lysine.

Jan 9, 2014 · Here we report new insights into the molecular mechanism of the regulatory interplay between the lysine-specific permease LysP and the membrane-integrated pH sensor CadC, which together induce lysine-dependent adaptation of E. coli under acidic stress.

Jan 4, 2019 · We find that the availability of external lysine is transduced to CadC via the co-sensor and inhibitor LysP, a lysine-specific transporter 12,13.

Aug 1, 1995 · Escherichia coli accumulates lysine via two systems, one specific for lysine (LysP) and a second inhibited by arginine or ornithine (LAO). The lysP gene encodes a polypeptide of 489 residues. A topological analysis of the LysP protein was performed using gene fusions. Random in-frame fusions of the lysP gene with the lacZ or blaM genes were ...

In this work, we describe the cloning, recombinant expression, purification, crystallization and preliminary diffraction from crystals of LysP from the human pathogen P. aeruginosa. The results reported here are a first step towards the determination of a high-resolution crystal structure of this important permease. 2. Materials and methods. 2.1.

Jan 1, 2014 · We report the heterologous production of the LysP-related transporter STM2200 from Salmonella typhimurium in Escherichia coli, its purification, and functional characterization. STM2200 is assumed to be a proton-dependent APC transporter of l-lysine.

The prokaryotic lysine-specific permease (LysP) belongs to the amino acid-polyamine-organocation (APC) transporter superfamily. In the cell, members of this family are responsible for the uptake and recycling of nutrients, for the maintenance of a constant internal ion concentration and for cell vol …

In contrast to Gram-negative bacteria, the lysP genes from Gram-positive bacteria have candidate LYS elements in their regulatory regions. At that, there are two and three lysP paralogs in the genomes of Lactococcus lactis and Clostridium acetobutylicum, respectively, but in each genome only one of them has an upstream LYS element.

Escherichia coli transports lysine by two distinct systems, one of which is specific for lysine (LysP) and the other of which is inhibited by arginine ornithine. The activity of the lysine-specific system increases with growth in acidic medium, anaerobiosis, and high concentrations of lysine.

May 17, 2024 · Here, we report the inward-occluded (3.2 – 5.3 Å) cryo-EM structure of Pseudomonas aeruginosa LysP bound to L-lysine and in complex with a nanobody. L–lysine is coordinated by hydrophobic stacking, cation-π interactions and hydrogen bonding mostly with polar uncharged LysP residues.