These mice may be useful in studying human neuronal alpha-synucleinopathies, such as familial Parkinson's Disease.</p>

The M83 transgenic mouse is a model of human synucleinopathies that develops severe motor impairment correlated with accumulation of the pathological Ser129-phosphorylated α-synuclein (α-syn^P ) in the brain and spinal cord. M83 disease can be accelerated by intracerebral inoculation of br …

2002年5月16日 · The spinal cord, cerebellum, and cortex of non-Tg (nTg) and Tg mice expressing wild-type (M7) and A53T (M83) human α-syn were sequentially extracted with buffers with increasing strength of protein solubilization.

2014年3月13日 · Results: Our data confirm our previous observations of disease acceleration in a transgenic mouse line (M83) overexpressing a mutated (A53T) form of human αS, following inoculation of either brain extracts from sick M83 mice or fibrillar recombinant αS.

2023年6月28日 · In this study, we aimed to visualize αSyn and iron deposits in the brains of a transgenic mouse model of PD, the M83 (A53T) line, using concurrent epifluorescence-vMSOT, high-field MRI, and scanning transmission X-ray microscopy. We utilized the novel pyrimidoindole derivative THK-565 for in vivo imaging of αSyn inclusions. 2. Methods. 2.1.

Homozygous M83 mice develop characteristic motor symptoms between 8 and 16 months of life, beginning with reduced ambulation, balance disorders, partial paralysis of a hind leg, and then progressing to prostration, difficulty in feeding, weight loss, hunched back and general paralysis (Giasson et al. 2002 ).

2014年3月13日 · We have now developed an ELISA test that specifically identifies the disease-associated αS (αS D) in brain homogenates prepared in High Salt buffer from sick M83 mice, without any concentration step, unlike Western blot that requires ultracentrifugation in the presence of sarkosyl to detect the protein (Additional file 1: Figure S1B) [14].

M83转基因小鼠是人类突触核蛋白病的模型，其发展为与脑和脊髓中病理性Ser129磷酸化α-突触核蛋白（α-synP）积累相关的严重运动障碍。 脑内接种患病M83小鼠的脑提取物可以加速M83疾病。

2024年4月27日 · 使用离体高场 T1 加权磁共振成像 (MRI) 以及 α-突触核蛋白 (磷酸化-S129) 和血管组织 (CD31 和 GLUT1) 的免疫染色来研究通过体内成像检测到的异常的性质。 离体分析表明，M83 小鼠脊髓中的血管网络并未受损。 体外 MRI 辅助基于深度学习的自动分割显示，与非转基因同窝小鼠相比，M83 小鼠的脊髓没有体积萎缩，而 Ser129 位点磷酸化的核 α-突触核蛋白可能与早期病理和代谢功能障碍有关。 所提出并经过验证的用于研究 PD 小鼠脊髓氧饱和度的非侵 …

2021年11月24日 · Following the administration of assembled A53T α-synuclein by either the oral or the nasal route, M83 ± mice developed severe motor abnormalities characterised by abnormal posture and gait, hindlimb dysfunction, inability to right and, eventually, paralysis.