2024年2月26日 · Here, we report a chemoproteomic method named ‘metal extraction-triggered agitation logged by thermal proteome profiling’ (METAL-TPP) to globally profile MBPs in proteomes. The method involves the...

2016年10月20日 · Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) which is properly expressed in E. coli might play a leading role and increase the expression levels of the target proteins. In this study, GAPDH was fused with a target enzyme, ChSase ABC I, an endoeliminase and polysaceharide lyase.

在重组蛋白中，常常将目的蛋白N端或C端与其他特定蛋白、多肽或寡肽标签进行融合表达，形成既能保留天然蛋白的大部分结构，又能实现增溶，防降解，促进分泌，便于纯化的功能；本文简要介绍生物药研发生产过程中常见的融合蛋白标签； 一、纯化标签. His标签是当前最流行的标签蛋白。 His标签是指六个组氨酸残基组成的融合标签，可插入在目的蛋白的C末端或N末端。 当某一个标签的使用，一是能构成表位利于检测；二是构成独特的结构特征（结合配体）利于纯化。 其特 …

2020年1月16日 · Here, we show for the first time that Gab1, an adaptor protein of receptor tyrosine kinase, is specifically expressed in OL lineage cells and is an essential effector of PDGF signaling in OPCs in mice. Gab1 is downregulated by PDGF stimulation and upregulated during OPC differentiation.

To test if the solubility-enhancement properties of these five mutations might extend to other proteins, we tested them as fusions to murine glyceraldehyde phosphate dehydrogenase (MBP-GAPDH), another protein whose solubility has been shown to …

2020年8月13日 · Using CRISPR-based gene editing we derived lines of mice bearing alleles deleted of one or more enhancers and these were assessed for Mbp and Golli mRNA accumulation (relative to Gapdh mRNA) at key stages of post-natal development. Each enhancer KO allele caused the greatest mRNA reduction in the …

2024年7月18日 · Myelin basic protein (Mbp) is essential for both elaboration and maintenance of CNS myelin, and its reduced accumulation results in hypomyelination. How different Mbp mRNA levels affect myelin dimensions across the lifespan and how resident glial cells may respond to such changes are unknown.

GAPDH was used to normalize to total protein levels. The graph represents densitometric analysis of the blots after 4 weeks of cuprizone treatment. Results (BDNF +/+ or BDNF +/− mice) are shown as a percentage of its own control (no cuprizone) GFAP/GAPDH. Data …

2024年6月5日 · Gapdh overexpression improved MBP production in TNF-α treated mature oligodendrocytes. To identify whether Gapdh is a critical part of myelin basic protein synthesis of mature oligodendrocyte during TNF-α damaging, we detected the expression of MBP mRNA and protein, as well as MYRF, a regulator of Mbp transcription [79].

2014年4月2日 · OPCs transfected with empty vector (NC) or GSK3β-S9A (S9A) for 2 days were lysed for immunoblotting with antibodies against MBP, CNP, pGSK3β, GSK3β, and GAPDH. All protein levels were normalized to the corresponding GAPDH level, and the percent changes are summarized in the bar graphs.